FoxH1 Antibody

Product Discontinued

FoxH1 Antibody Summary

• Immunogen: Synthetic peptide: GPCSGSRLGPPEAE representing the N-Terminus of the human protein (residues 2-15), according to NP_003914.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXH1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:8000
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave no signal but low background in Human Skeletal Muscle and A431 lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
• Control:

  FoxH1 Overexpression Lysate

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for FoxH1 Antibody

• FAST1
• Fast-1
• FAST1forkhead box protein H1
• FAST2
• Fast-2
• Forkhead activin signal transducer 1
• Forkhead activin signal transducer 2
• forkhead activin signal transducer-1
• forkhead box H1
• FoxH1
• hFAST-1
• TGF-beta/activin signal transducer

Background

Fas, also referred to as CD95 or APO-1, is a type I transmembrane protein that plays a central role mediating viral immunity. TIA-1 and TIAR are two closely related proteins that possess three RRMs (RNA recognition motifs), designated RRM 1, 2 and 3, respectively. Although both TIA-1 and TIAR are thought to function as mediators of apoptotic cell death, their specific roles in such pathways are unknown. Unlike TIA-1, which is found in the granules of cytotoxic lymphocytes, TIAR expression is limited to the nucleus and found in a much broader range of cells including, but not limited to, cells of hematopoietic origin. TIAR is translocated to the cytoplasm shortly after Fas ligation and this event immediately proceeds the onset of DNA fragmentation. A novel serine/ threonine kinase that is activated as a result of Fas ligation, designated FAST (Fas-activated serine/threonine), shows kinase specificity towards both TIA-1 and TIAR. In unstimulated Jurkat cells, FAST resides in the cytoplasm as a highly phosphorylated protein and is quickly dephosphorylated and activated in response to stimulated Fas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXH1
• Entrez:
  • 8928(Human)
• Uniprot:
  • NP_003914.(Human)