FLIP Antibody


Western Blot: FLIP Antibody [NBP1-76972] - Analysis of FLIP g/ d in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with anti-FLIP g/ d (CT) using NBP1-76972 at 1:1000 ...read more
Immunocytochemistry/ Immunofluorescence: FLIP Antibody [NBP1-76972] - Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: FLIP Antibody [NBP1-76972] - Analysis of formalin-fixed HeLa cells using anti-FLIP g/ d (CT) at 2 ug/ml.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
1 mg/ml

Order Details

FLIP Antibody Summary

Antibody was raised against a peptide corresponding to amino acids 191 to 209 at C-terminus of human FLIP form2.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
FLIP can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kDa and 25 kDa bands can be detected. antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.
Control Peptide
FLIP Peptide (NBP1-76972PEP)

Reactivity Notes


Alternate Names for FLIP Antibody

  • CASHFADD-like antiapoptotic molecule 1
  • CASP8 and FADD-like apoptosis regulator
  • CASP8AP1
  • Caspase homolog
  • Caspase-eight-related protein
  • Caspase-like apoptosis regulatory protein
  • caspase-related inducer of apoptosis
  • CasperFLAME1
  • Cellular FLICE-like inhibitory protein
  • c-FLIPR
  • c-FLIPS
  • CLARPMACH-related inducer of toxicity
  • FADD-like apoptotic molecule
  • FLAME-1
  • Inhibitor of FLICE
  • usurpin beta
  • Usurpin


Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively. The human FLIPs were also cloned by several labs independently and termed Casper I-FLICE, FLAME-1, CASH, CLARP and usurpin. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on FLIP.

TRAIL-R2: The Trail Less Traveled
Cells undergo apoptotic programmed cell death in response to various stimuli, and this key mechanism is necessary for cellular morphogenesis, tissue homeostasis, and host defense. Particular cytokines such as tumor necrosis factor (TNF) and the Fas li...  Read full blog post.

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Product PDFs


Gene Symbol CFLAR

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