Flightless I Antibody

Images

 
Western Blot: Flightless I Antibody [NBP2-16501] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Flightless I Antibody [NBP2-16501] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Western Blot: Flightless I Antibody [NBP2-16501] - 50 ug rat muscle lysate/extract 5% SDS-PAGE Flightless I antibody [C2C3], C-term dilution: 1:1000.
Western Blot: Flightless I Antibody [NBP2-16501] - Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.95 mg/ml

Order Details

Flightless I Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Flightless I. The exact sequence is proprietary.
Localization
Nucleus (By similarity); Cytoplasm; cytoskeleton (By similarity); Centrosome (By similarity)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FLII
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (81%), Chicken (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.95 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Flightless I Antibody

  • FLI
  • Fli1
  • flightless I homolog (Drosophila)
  • FLILflightless I (Drosophila) homolog
  • MGC39265
  • protein flightless-1 homolog

Background

This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Flightless I Antibody (NBP2-16501) (0)

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol FLII
Uniprot