Flightless I Antibody

Images

 
Western Blot: Flightless I Antibody [NBP1-31610] - Sample(30 ug whole cell lysate)A:Raji 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Flightless I Antibody [NBP1-31610] - Paraformaldehyde-fixed mouse ESC D3, using antibody at 1:200 dilution.
Western Blot: Flightless I Antibody [NBP1-31610] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Flightless I Antibody [NBP1-31610] - Paraformaldehyde-fixed HeLa, using Flightless I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.88 mg/ml

Order Details

Flightless I Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Flightless I. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm, cytoskeleton, Centrosome
Specificity
This antibody is specific for FLI1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FLII
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Chicken (87%) and Xenopus tropicalis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.88 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Flightless I Antibody

  • FLI
  • Fli1
  • flightless I homolog (Drosophila)
  • FLILflightless I (Drosophila) homolog
  • MGC39265
  • protein flightless-1 homolog

Background

FLI1 is a member of ETS family of transcription factors and is a proto-oncogene involved in erythroleukemia and Ewings sarcoma. FLI1 was originally identified as a common proviral insertion (Friend leukemia insertion site 1) and found in 75% of erythroleukemia cell clones induced by the Friend mouse leukemia virus. A chromosomal translocation between the FLI1 gene and EWS gene is found in most Ewing sarcomas. FLI1 has anti-apoptotic acivities and also interferes with nuclear hormone receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLII
Entrez
Uniprot