Western Blot: FliC Antibody (FLIC-1) [NBP1-48718] - Recombinant flagellin protein (50 ng per lane) was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against FliC. ...read more
The immunogen was the full length version of the protein: FliC (FLIC-1)
Expressed in many motile enteric bacteria including Salmonella and Escherichia
FliC is expressed in motile bacteria including Salmonella and E. Coli. The epitope recognized by this antibody is not known. There is a possibility that some flagellins are not recognized by this FLIC-1 antibody.
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 ug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each applicationEach lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Read Publications using NBP1-48718 in the following applications:
FliC is a protein expressed in many motile enteric bacteria including Salmonella and Escherichia. The C- and N-terminal regions of the protein among various strains of bacteria are well-conserved. However, there is great variability of length and amino acid sequence in the central region. For example, E. coli flagellin have been reported to vary in size from 36 K to 69 K in MW. The epitope recognized by this FLIC-1 monoclonal antibody is not known. FliC is a subunit protein that polymerizes (in conjunction with other proteins) to form the filaments of bacterial flagella in a precise order. Flagellin is a potent ligand of toll-like receptor 5 (TLR5). By binding to TLR5, flagellin induces activation of NF-kB and triggers the production of cytokines and innate immune responses.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for FliC Antibody (NBP1-48718)(6)
We have publications tested in 1 confirmed species: Bacteria.
FAQs for FliC Antibody (NBP1-48718). (Showing 1 - 2 of 2 FAQs).
Do you know if the antibody reacts with flagellin from S. Typhimuriom?
The epitope recognized by this FLIC-1 monoclonal antibody is not known and unfortunately, I do not have access to the inventor's publications on the development / characterization of this antibody or its testing on Salmonella typhimuriom. However, based on the data shown in the following publications, it appears that this antibody should be good for detection of FLIC in Salmonella typhimuriom strains. (1) Knodler et al. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17733-8 (2) Uchiya and Nikai. Microbiology. 2008 Nov;154(Pt 11):3491-502. See sgmjournals site
Do you know which species the antibody was generated against?
The immunogen for this product was derived from the C-terminal region of E. coli.