FliC Antibody (FLIC-1)

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Western Blot: FliC Antibody (FLIC-1) [NBP1-48718] - Recombinant flagellin protein (50 ng per lane) was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody against FliC. ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-48718
    • Availability
      Product Discontinued

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FliC Antibody (FLIC-1) Summary

Immunogen
The immunogen was the full length version of the protein: FliC (FLIC-1)
Localization
Expressed in many motile enteric bacteria including Salmonella and Escherichia
Specificity
FliC is expressed in motile bacteria including Salmonella and E. Coli. The epitope recognized by this antibody is not known. There is a possibility that some flagellins are not recognized by this FLIC-1 antibody.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 ug antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each applicationEach lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Publications
Read Publications using
NBP1-48718 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for FliC Antibody (FLIC-1)

  • Flagellin component FliC

Background

FliC is a protein expressed in many motile enteric bacteria including Salmonella and Escherichia. The C- and N-terminal regions of the protein among various strains of bacteria are well-conserved. However, there is great variability of length and amino acid sequence in the central region. For example, E. coli flagellin have been reported to vary in size from 36 K to 69 K in MW. The epitope recognized by this FLIC-1 monoclonal antibody is not known. FliC is a subunit protein that polymerizes (in conjunction with other proteins) to form the filaments of bacterial flagella in a precise order. Flagellin is a potent ligand of toll-like receptor 5 (TLR5). By binding to TLR5, flagellin induces activation of NF-kB and triggers the production of cytokines and innate immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FliC Antibody (NBP1-48718)(6)

We have publications tested in 1 confirmed species: Bacteria.

We have publications tested in 1 application: WB.


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WB
(1)
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Bacteria
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Showing Publications 1 - 6 of 6.
Publications using NBP1-48718 Applications Species
Chang KC, Cheng SJ, Chen YC et al. Nanoscopic analysis on pH induced morphological changes of flagella in Escherichia coli J Microbiol Immunol Infect 2012 Sep 24 [PMID: 23017690] (WB, Bacteria) WB Bacteria
Knodler LA, Vallance BA, Celli J, Winfree S, Hansen B, Montero M, Steele-Mortimer O. Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia. Proc Natl Acad Sci U S A 2010 Oct 12. [PMID: 20876119]
Crawford RW, Reeve KE, Gunn JS. Flagellated but not hyperfimbriated Salmonella enterica serovar Typhimurium attaches to and forms biofilms on cholesterol-coated surfaces. J Bacteriol;192(12):2981-90. 2010 Jun. [PMID: 20118264]
Ibarra JA, Knodler LA, Sturdevant DE, Virtaneva K, Carmody AB, Fischer ER, Porcella SF, Steele-Mortimer O S. nduction of Salmonella pathogenicity island 1 under different growth conditions can affect Salmonella-host cell interactions in vitro. Microbiology;156(Pt 4):1120-33. 2010 Apr. [PMID: 20035008]
Huang LY, Dumontelle JL, Zolodz M, Deora A, Mozier NM, Golding B. Use of toll-like receptor assays to detect and identify microbial contaminants in biological products. J Clin Microbiol;47(11):3427-34. 2009 Nov. [PMID: 19726599]
Uchiya K, Nikai T. Salmonella virulence factor SpiC is involved in expression of flagellin protein and mediates activation of the signal transduction pathways in macrophages. Microbiology. 154(Pt 11):3491-502. 2008 Nov. [PMID: 18957602]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FliC Antibody (NBP1-48718). (Showing 1 - 2 of 2 FAQs).

  1. Do you know if the antibody reacts with flagellin from S. Typhimuriom?
    • The epitope recognized by this FLIC-1 monoclonal antibody is not known and unfortunately, I do not have access to the inventor's publications on the development / characterization of this antibody or its testing on Salmonella typhimuriom. However, based on the data shown in the following publications, it appears that this antibody should be good for detection of FLIC in Salmonella typhimuriom strains. (1) Knodler et al. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17733-8 (2) Uchiya and Nikai. Microbiology. 2008 Nov;154(Pt 11):3491-502. See sgmjournals site
  2. Do you know which species the antibody was generated against?
    • The immunogen for this product was derived from the C-terminal region of E. coli.

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