FKBP52/FKBP4 Antibody (4D3)

Images

 
Western Blot: FKBP4/FKBP52 Antibody (AT4D3) [NBP1-30162] - Cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FKBP4 (1:1000). Proteins were visualized using a ...read more
Immunohistochemistry-Paraffin: FKBP4/FKBP52 Antibody (AT4D3) [NBP1-30162] - Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
4D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FKBP52/FKBP4 Antibody (4D3) Summary

Immunogen
Recombinant human FKBP4 (1-459aa) purified from E. coli (NP_002005).
Specificity
This antibody is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FKBP4 protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FKBP4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
For IHC: Antigen retrieval in 0.1M sodium citrate buffer and detection using Diaminobenzidine (DAB) is recommended.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-30162.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FKBP52/FKBP4 Antibody (4D3)

  • 51 kDa FK506-binding protein
  • 52 kDa FKBP
  • 59 kDa immunophilin
  • EC 5.2.1.8
  • FK506 binding protein 4, 59kDa
  • FK506-binding protein 4 (59kD)
  • FK506-binding protein 4
  • FKBP4
  • FKBP-4
  • FKBP51
  • FKBP52
  • FKBP-52
  • FKBP52T-cell FK506-binding protein, 59kD
  • FKBP59
  • FKBP5952 kDa FK506-binding protein
  • HBI
  • HSP binding immunophilin
  • Hsp56
  • HSP-binding immunophilin
  • Immunophilin FKBP52
  • p52
  • p59
  • peptidyl-prolyl cis-trans isomerase FKBP4
  • peptidylprolyl cis-trans isomerase
  • PPIase FKBP4
  • PPIase
  • rotamase

Background

FKBP4, also known as FKBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. Recombinant human FKBP4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FKBP4/FKBP52 Antibody (NBP1-30162)(1)

We have publications tested in 1 confirmed species: Human.


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Bioinformatics

Gene Symbol FKBP4
Entrez
Uniprot