FITC Antibody (2A3)

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Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, S-ELISA, Cell Depl
Clone
2A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

FITC Antibody (2A3) Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human soluble CD40 Ligand / TRAP Analog conjugates with BSA.
Specificity
This product reacts with both free fluorescein (FITC) and conjugated FITC, so it recognizes FITC-labeled proteins. Kd = 1.5 x 10^-8 M
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Sandwich ELISA
  • Cell depletion
Publications
Read Publication using NB110-7898.

Reactivity Notes

Detects FITC labelled products.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for FITC Antibody (2A3)

  • FITC
  • Fluorescein Isothiocyanate

Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FITC Antibody (NB110-7898)(1)

We have publications tested in 1 application: Cell Depl.


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Cell Depl
(1)
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Publication using NB110-7898 Applications Species
Method to deplete carryover white blood cells from CELLSEARCH (R) Epithelial Cell Profile Kit samples Technical Notes (Cell Depl) Cell Depl

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Product General Protocols

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Video Protocols

WB Video Protocol

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