Recombinant Human Fibulin 1 Protein

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SDS-Page: Fibulin 1 Recombinant Protein [H00002192-P01]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PA

Order Details

Recombinant Human Fibulin 1 Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-683 of Human FBLN1 full-length ORF

Source: Wheat Germ (in vitro)

Amino Acid Sequence:MERAAPSRRVPLQLLLLGGLALLAAGVDADVLLEACCADGHRMATHQKDCSLPYATESKECRMVQEQCCHSQLEELHCATGISLANEQDRCATPHGDNASLEATFVKRCCHCCLLGRAAQAQGQSCEYSLMVGYQCGQVFRACCVKSQETGDLDVGGLQETDKIIEVEEEQEDPYLNDRCRGGGPCKQQCRDTGDEVVCSCFVGYQLLSDGVSCEDVNECITGSHSCRLGESCINTVGSFRCQRDSSCGTGYELTEDNSCKDIDECESGIHNCLPDFICQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPIGHTCINTEGSYTCQKNVPNCGRGYHLNEEGTRCVDVDECAPPAEPCGKGHRCVNSPGSFRCECKTGYYFDGISRMCVDVNECQRYPGRLCGHKCENTLGSYLCSCSVGFRLSVDGRSCEDINECSSSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALPTGGHICSYRCINIPGSFQCSCPSSGYRLAPNGSNCQDIDECVTGIHNCSINETCFNIQGGFRCLAFECPENYRRSAATRCERLPCHENRECSKLPLRITYYHLSFPTNIQAPAVVFRMGPSSAVPGDSMQLAITGGNEEGFFTTRKVSPHSGVVALTKPVPEPRDLLLTVKMDLSRHGTVSSFVAKLFIFVSAEL

Protein/Peptide Type
Recombinant Protein
Gene
FBLN1

Applications/Dilutions

Application Notes
This protein has not been tested for any functionality. Product may contain endotoxins and is not suitable for use with live cells.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Fibulin 1 Protein

  • FBLN
  • FBLN1
  • FIBL1
  • FIBL-1
  • Fibulin 1
  • fibulin-1

Background

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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WB Video Protocol

FAQs for Fibulin 1 Recombinant Protein (H00002192-P01). (Showing 1 - 2 of 2 FAQ).

  1. Hi, I would like to know more about the Human FBLN-1 recombinant protein. What cell expression system was used to obtain the protein, and also, how much is freight to Melbourne Australia? Your response will be greatly appreciated. Thank you
    • This protein is produced by a Taiwanese company called Abnova and we distribute it for them. Their proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots as a positive control with their antibody. As far as pricing to Australia, you will need to contact Sapphire Bioscience, our Australian distributor.
  2. I'm interested in your Fibulin 1 Recombinant Protein (H00002192-P01); however, on the datasheet I cannot find which is the source for this recombinant (E.coli, Yeast, etc)? Also, could you confirm that this recombinant correspond to a Fibulin 1 isoform C?
    • These products you are interested in are products we distribute for Abnova. We therefore do not preform any in-house testing or development of their products. The Fibulin 1 recombinant protein was raised in a cell free wheat germ system. It was made to the full length isoform C Fibulin 1 protein. I will include a link to Abnova's description of the cell free wheat germ system.

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Bioinformatics

Gene Symbol FBLN1
Entrez