Fibronectin/Anastellin Antibody


Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Fibronectin/Anastellin Antibody [NBP1-31369] - Analysis of HeLa, using NBP1-31369 at 1:200 dilution.
Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-31369] - Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.
Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Analysis of NBP1-31369 on Hela, with 5% SDS PAGE, diluted at 1:1000.
Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Whole cell extracts (30 ug) was separated by 5 % SDS-PAGE, and blotted with Fibronectin antibody [N1N2], N-term diluted by 1:1000
Immunoprecipitation: Fibronectin/Anastellin Antibody [NBP1-31369] - N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of more

Product Details

Reactivity Hu, Mu, Bovine, RatSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

Order Details

Fibronectin/Anastellin Antibody Summary

Recombinant fragment corresponding to a region within amino acids 396 and 689 of Fibronectin (Uniprot ID#P02751)
Secreted, extracellular space, extracellular matrix
Mesenchymal Cells Marker
Predicted Species
Rat (92%), Bovine (92%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.0) and 20% Glycerol
0.025% Proclin 300
1.0 mg/ml
Immunogen affinity purified


  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
Immunofluorescence, Western blot. The usefulness of this product in other applications has not been determined.
Theoretical MW
263 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken 88%.

Alternate Names for Fibronectin/Anastellin Antibody

  • CIGDKFZp686O13149
  • Cold-insoluble globulin
  • DKFZp686F10164
  • DKFZp686H0342
  • DKFZp686I1370
  • fibronectin 1
  • fibronectin
  • FNZ
  • migration-stimulating factor
  • MSF


Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibronectin/Anastellin Antibody (NBP1-31369). (Showing 1 - 2 of 2 FAQ).

  1. One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?
    • Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P
  2. For NBP1-31369 the Fibronectin antibody, do I need to add any other buffer solution before aliquotting it?
    • For the Fibronectin antibody (NBP1-31369) you do not need to add anything else to the buffer to store it in your aliquots. It already has glycerol, which will keep it stable at lower temperatures and the main thing is to avoid repeated freeze thaw cycles that might cause your antibody to lose binding affinity.

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol FN1

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