Fibronectin/Anastellin Antibody (SPM539) [DyLight 405LS]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM539
Clonality
Monoclonal
Host
Mouse

Fibronectin/Anastellin Antibody (SPM539) [DyLight 405LS] Summary

Immunogen
Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose
Localization
Connective tissue matrix
Specificity
Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 405LS
Format
DyLight 405LS (A=397, E=572)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Alternate Names for Fibronectin/Anastellin Antibody (SPM539) [DyLight 405LS]

  • CIGDKFZp686O13149
  • Cold-insoluble globulin
  • DKFZp686F10164
  • DKFZp686H0342
  • DKFZp686I1370
  • fibronectin 1
  • fibronectin
  • Fibronectin/Anastellin
  • FNFINC
  • FNZ
  • GFND2ED-B
  • LETSGFND
  • migration-stimulating factor
  • MSF

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibronectin/Anastellin Antibody (NBP2-34785V3). (Showing 1 - 1 of 1 FAQ).

  1. One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?
    • Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol FN1
Entrez

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