Fibrinogen Antibody [HRP]


Western Blot: Fibrinogen Antibody [HRP] [NB600-927] - Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4C. Secondary more
Immunohistochemistry-Paraffin: Fibrinogen Antibody [HRP] [NB600-927] - Human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
1.0 mg/ml

Order Details

Fibrinogen Antibody [HRP] Summary

This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Fibrinogen [Human Plasma]
This recognizes human fibrinogen, a 340kD glycoprotein which plays an important role in the coagulation process. A 4-8% gradient gel is recommended under non-reducing conditions. Under reducing conditions, bands maybe be seen at 63.5, 56 and 47 kDa.
Delipidation and Defibrination
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
0.01% Gentamicin Sulfate
1.0 mg/ml
Delipidation and Defibrination


Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Alternate Names for Fibrinogen Antibody [HRP]

  • Factor I
  • FGA
  • FGB
  • Fib2
  • fibrinogen alpha chain
  • fibrinogen, A alpha polypeptide
  • MGC119422
  • MGC119423
  • MGC119425


Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel A. et al, and Rapold H.J. et al). All these facts make fibrinogen an important parameter in the diagnosis of cardiovascular diseases.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies


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Gene Symbol FGA

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