FGF R3 Antibody (136312) [Unconjugated] - (IIIc)

Images

 
FGF R3 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human FGF R3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
FGF R3 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Mouse Anti-Human FGF R3 (IIIc) Monoclonal Antibody (Catalog # MAB7662) at 25 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
136312
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

FGF R3 Antibody (136312) [Unconjugated] - (IIIc) Summary

Additional Information
Clone 136312 was used by HLDA to establish CD designation
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIc)
Specificity
Detects human FGF R3 (IIIc) in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with with recombinant human (rh) FGF R2 (IIIc), recombinant mouse (rm) FGF R2 (IIIc) and rmFGF R3 (IIIc) is observed. In Western blots (non-reducing conditions only), 50‑100% cross-reactivity with rhFGF R2 (IIIc), rmFGF R2 (IIIc) and rmFGF R3 (IIIc) is observed. Does not cross-react with the IIIb isoforms of rhFGF R3, rmFGF R3, rhFGF R2 or rmFGF R2. No cross-reactivity with any isoforms of rhFGF RI or rhFGF R4 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Positive Control
FGF R3 Lysate (NBP2-65023)
Reviewed Applications
Read 1 Review rated 3
using
MAB7662 in the following applications:

Publications
Read Publication using
MAB7662 in the following applications:

  • IP
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF R3 Antibody (136312) [Unconjugated] - (IIIc)

  • CD333
  • CEK
  • EC 2.7.10
  • FGF R3
  • FGFR3
  • fibroblast growth factor receptor 3
  • HSFGFR3EX
  • JTK4
  • JTK4thanatophoric dwarfism

Background

FGF R3 is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co-factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF-1, -2, -4, -8b, -8e, -8f, -9 and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF R3 Antibody (MAB7662)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


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IP
(1)
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Human
(1)
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Review for FGF R3 Antibody (MAB7662) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB7662:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry FGF R3 MAB7662
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reviewed by:
Natalie Erdmann
ICC Human 01/26/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedKMS11, LP-1 and RPMI8226 multiple myeloma cell lines
SpeciesHuman
LotGHK051512A

Blocking

Blocking Details10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

Dilution Ratio1/30 1ary antibody diluted in 10% donkey serum/PBS overnight at 4oC

Secondary Antibody

Secondary DescriptionDonkey F(ab')2 anti-mouse IgG (H+L) Alexa Fluor 488
Secondary Manufacturer Cat#Jackson 711-546-150
Secondary Concentration1/1000

Details

Detection NotesZeiss AxioImager, FITC filter cube for 300 ms exposure
Fixation DetailsImmersion-fixed in 4% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
Wash Description3X 5 minutes PBS after each step

Comments

CommentsWeak staining in positive control KMS11, negative controls fine with minimal background

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FGF R3 Antibody (MAB7662) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for FGF R3 Antibody (MAB7662)

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Recent Reviews

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1
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Natalie Erdmann
01/26/2016
Application: ICC
Species: Human

Bioinformatics

Gene Symbol FGFR3
Entrez