FGF R2 Antibody (3F8)


Western Blot: FGFR2 Antibody (3F8) [NBP2-00670] - Staining of paraffin-embedded Human breast tissue using anti-FGFR2 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: FGFR2 Antibody (3F8) [NBP2-00670] Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2.
Immunohistochemistry-Paraffin: FGFR2 Antibody (3F8) [NBP2-00670] - Staining of paraffin-embedded Human tonsil using anti-FGFR2 mouse monoclonal antibody.
Flow Cytometry: FGFR2 Antibody (3F8) [NBP2-00670] - Analysis of Hela cells, using anti-FGFR2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: FGFR2 Antibody (3F8) [NBP2-00670] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: FGFR2 Antibody (3F8) [NBP2-00670] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FGFR2 Antibody (3F8) [NBP2-00670] - Staining of paraffin-embedded Human Kidney tissue using anti-FGFR2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FGFR2 Antibody (3F8) [NBP2-00670] - Staining of paraffin-embedded Human lymphoma tissue using anti-FGFR2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FGFR2 Antibody (3F8) [NBP2-00670] - Staining of paraffin-embedded Human pancreas tissue using anti-FGFR2 mouse monoclonal antibody.

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FGF R2 Antibody (3F8) Summary

Full length human recombinant protein of human FGFR2(NP_000132) produced in HEK293T cell.
Immunogen affinity purified
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  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for FGF R2 Antibody (3F8)

  • BBDS
  • BEK
  • BFR-1
  • CD332 antigen
  • CD332
  • CEK3
  • CFD1
  • craniofacial dysostosis 1
  • EC 2.7.10
  • EC
  • ECT1
  • FGF R2
  • FGFR2
  • fibroblast growth factor receptor 2
  • FLJ98662
  • Jackson-Weiss syndrome
  • JWS
  • Keratinocyte growth factor receptorreceptor like 14
  • KGFR
  • KSAM
  • K-sam
  • soluble FGFR4 variant 4
  • TK14
  • TK25


The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
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FAQs for FGF R2 Antibody (NBP2-00670). (Showing 1 - 2 of 2 FAQ).

  1. Do you have any data to demonstrate the specificity of this antibody? For example, have you tested the antibody on any known negative cell lines or tissues to confirm there is no cross-reactivity with other isoforms or proteins?
    • We have not tested this antibody against known negative tissues or against cell lines known to express other isoforms. We do, however, have a Western blot on the antibody's datasheet where HEK293T cells transfected with empty vector gave no signal, but we are not sure what the expression pattern of this cell line is.
  2. I had a question about your FGFR2 antibody. I know that FGFR2 is highly conserved among species and I was wondering what the cross reactivity of this antibody is? It is tested for Flow, but if I were using it in a mixture of human and murine cells, should I expect cross reactivity even though it is specified for human FGFR2?
    • In regards to your question regarding our FGFR2 antibody (NBP2-00670), it is very likely that there will be cross reaction of this antibody with the mouse protein. I have aligned the mouse and human sequences for this protein. We do not list mouse as a reactive species only because we have not yet tested for this cross reactivity. Based on the alignment of the two protein, it looks like there is a high chance that the antibody would detect the mouse protein.

Secondary Antibodies


Isotype Controls

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Gene Symbol FGFR2