FGF R1 Antibody (M2F12)

Images

 
Western Blot: FGF R1 Antibody (M2F12) [NB120-829] - Total protein from Ntera-2 was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/ml of anti-FGFR1. FGFR1 ...read more
Immunocytochemistry/ Immunofluorescence: FGF R1 Antibody (M2F12) [NB120-829] - NTERA2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in MCF-7 cells (1 x 10^6 cells/ml) stained with FGF r1 antibody (NB120-829, red peaks) at a 1:1000 dilution. Detected with a FITC conjugated goat ...read more
Flow Cytometry: FGF R1 Antibody (M2F12) [NB120-829] - Analysis of FGF R1 in HEK293 cells (2 x10^6) stained with FGF R1 antibody (NB120-829, red) at a 1:1000 dilution and detected with a FITC conjugated goat anti-mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IP, CyTOF-ready
Clone
M2F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FGF R1 Antibody (M2F12) Summary

Immunogen
Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.
Specificity
Reacts with alpha isoform of FRFR1. Reacts with the NH2-terminus of unique NH2-terminal Ig loop of FGFr1.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FGFR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculo-viral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Customers have reported difficulties using this antibody in immunocytochemistry staining (see review).
Publications
Read Publications using
NB120-829 in the following applications:

  • IHC
    2 publications
  • WB
    1 publication

Reactivity Notes

Reacts with human, rat, and mouse receptor. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FGF R1 Antibody (M2F12)

  • basic fibroblast growth factor receptor 1
  • BFGFR
  • bFGF-R-1
  • CD331 antigen
  • CD331
  • CEK
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R1
  • FGFBR
  • FGFR1
  • FGFR-1
  • fibroblast growth factor receptor 1
  • FLGH3
  • FLJ99988
  • Flt-2
  • FLT2H4
  • Fms-like tyrosine kinase 2
  • fms-related tyrosine kinase 2
  • H2
  • H5
  • HBGFR
  • heparin-binding growth factor receptor
  • hydroxyaryl-protein kinase
  • KAL 2
  • KAL2
  • N-SAM
  • OGD
  • Proto-oncogene c-Fgr
  • soluble FGFR1 variant 1
  • soluble FGFR1 variant 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF R1 Antibody (NB120-829)(3)

Reviews for FGF R1 Antibody (NB120-829) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FGF R1 Antibody (NB120-829). (Showing 1 - 2 of 2 FAQs).

  1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
    • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
  2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
    • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-829AF405
Alexa Fluor 488 Labeled NB120-829AF488
Alexa Fluor 647 Labeled NB120-829AF647
Alexa Fluor 700 Labeled NB120-829AF700
Allophycocyanin Labeled NB120-829APC
Biotin Labeled NB120-829B
DyLight 350 Labeled NB120-829UV
DyLight 405 Labeled NB120-829V
DyLight 488 Labeled NB120-829G
DyLight 550 Labeled NB120-829R
DyLight 650 Labeled NB120-829C
DyLight 680 Labeled NB120-829FR
DyLight 755 Labeled NB120-829IR
HRP Labeled NB120-829H
PE Labeled NB120-829PE
PerCP Labeled NB120-829PCP

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Bioinformatics

Gene Symbol FGFR1
Entrez