FGF R1 Antibody (M19B2) [Alexa Fluor® 488]

Images

 
Flow Cytometry: FGF R1 Antibody (M19B2) [Alexa Fluor (R) 488] [NB600-1287AF488] - analysis of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. Red - positive cells, Green - negative cells, ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow
Clone
M19B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

FGF R1 Antibody (M19B2) [Alexa Fluor® 488] Summary

Immunogen
Recombinant human ectodomain of FGFR1b expressed in E. coli beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
Epitope is within the NH2-terminus of Ig loop II sequence lys145 and glu159 between the acidic box and heparin-binding sequence of FGFR1.
Localization
Cell Membrane
Specificity
Reacts with both alpha and beta isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
Application Notes
May be useful in Flow Cytometry as reported in reviews.
Reviewed Applications
Read 1 Review rated 4
using
NB600-1287AF488 in the following applications:

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FGF R1 Antibody (M19B2) [Alexa Fluor® 488]

  • basic fibroblast growth factor receptor 1
  • BFGFR
  • bFGF-R-1
  • CD331 antigen
  • CD331
  • CEK
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R1
  • FGFBR
  • FGFR1
  • FGFR-1
  • fibroblast growth factor receptor 1
  • FLGH3
  • FLJ99988
  • Flt-2
  • FLT2H4
  • Fms-like tyrosine kinase 2
  • fms-related tyrosine kinase 2
  • H2
  • H5
  • HBGFR
  • heparin-binding growth factor receptor
  • hydroxyaryl-protein kinase
  • KAL 2
  • KAL2
  • N-SAM
  • OGD
  • Proto-oncogene c-Fgr
  • soluble FGFR1 variant 1
  • soluble FGFR1 variant 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF R1 Antibody (NB600-1287AF488) (0)

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Review for FGF R1 Antibody (NB600-1287AF488) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NB600-1287AF488:
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Flow
(1)
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(1)
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Images Ratings Applications Species Date Details
Flow Cytometry FGF R1 NB600-1287AF488
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reviewed by:
Anonymous
Flow Human 05/01/2015
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Summary

ApplicationFlow Cytometry
Sample TestedHEK-293 and HEK-293 FGFR transfected cells
SpeciesHuman
LotA-1-AF488

Blocking

Blocking Details1% FBS in DPBS with Ca and Mg for 30 min, room temperature

Primary Anitbody

Dilution Ratio1 ul of Ab per 100 ul of sample (300 000 cells), incubation 90 min, room temperature, DPBS buffer with Ca and Mg

Details

Detection NotesBeckam Gallios flow cytometer
Fixation DetailsCells were resuspended in DPBS buffer with Ca and Mg
Permeabilization DetailsNo permeabilization
Wash Description2 washing cycles

Comments

CommentsAs it can be seen from the figure there was some binding to negative cells. Probably, a different blocking agent should be used to eliminate this problem.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FGF R1 Antibody (NB600-1287AF488). (Showing 1 - 2 of 2 FAQ).

  1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
    • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
  2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
    • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NB600-1287AF647
Alexa Fluor 700 Labeled NB600-1287AF700

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Recent Reviews

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Anonymous
05/01/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol FGFR1
Entrez