FGF R1 Antibody (M19B2)

Images

 
Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Western blot analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor (R) 488 conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. Red - ...read more
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
M19B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FGF R1 Antibody (M19B2) Summary

Immunogen
Recombinant human ectodomain of FGFR1b expressed in E. coli beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
Epitope is within the NH2-terminus of Ig loop II sequence lys145 and glu159 between the acidic box and heparin-binding sequence of FGFR1.
Localization
Cell Membrane
Specificity
Reacts with both alpha and beta isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1 ug per million cells
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Reviewed Applications
Read 1 Review rated 5
using
NB600-1287 in the following applications:

Publications
Read Publications using
NB600-1287 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FGF R1 Antibody (M19B2)

  • basic fibroblast growth factor receptor 1
  • BFGFR
  • bFGF-R-1
  • CD331 antigen
  • CD331
  • CEK
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R1
  • FGFBR
  • FGFR1
  • FGFR-1
  • fibroblast growth factor receptor 1
  • FLGH3
  • FLJ99988
  • Flt-2
  • FLT2H4
  • Fms-like tyrosine kinase 2
  • fms-related tyrosine kinase 2
  • H2
  • H5
  • HBGFR
  • heparin-binding growth factor receptor
  • hydroxyaryl-protein kinase
  • KAL 2
  • KAL2
  • N-SAM
  • OGD
  • Proto-oncogene c-Fgr
  • soluble FGFR1 variant 1
  • soluble FGFR1 variant 2

Background

FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF R1 Antibody (NB600-1287)(3)

Review for FGF R1 Antibody (NB600-1287) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB600-1287:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Lisa Renshaw
Flow Other 12/16/2010
View

Summary

ApplicationFlow Cytometry
Sample Testedtransfected cell line
SpeciesOther
Commentswe get great FACS results with Novus antibodies

Comments

Commentswe get great FACS results with Novus antibodies

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FGF R1 Antibody (NB600-1287). (Showing 1 - 2 of 2 FAQs).

  1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
    • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
  2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
    • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1287AF405
Alexa Fluor 488 Labeled NB600-1287AF488
Alexa Fluor 647 Labeled NB600-1287AF647
Alexa Fluor 700 Labeled NB600-1287AF700
Allophycocyanin Labeled NB600-1287APC
DyLight 350 Labeled NB600-1287UV
DyLight 405 Labeled NB600-1287V
DyLight 488 Labeled NB600-1287G
DyLight 680 Labeled NB600-1287FR
DyLight 755 Labeled NB600-1287IR
PE Labeled NB600-1287PE
PerCP Labeled NB600-1287PCP

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Bioinformatics Tool for FGF R1 Antibody (NB600-1287)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Lisa Renshaw
12/16/2010
Application: Flow
Species: Other

Bioinformatics

Gene Symbol FGFR1
Entrez
Uniprot