FGF R1 Antibody (M17A3)

Images

 
Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml ...read more
Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat ...read more
Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, IHC-P, IP, CyTOF-ready
Clone
M17A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FGF R1 Antibody (M17A3) Summary

Immunogen
Recombinant human ectodomain of FGF R1 expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
Epitope is undetermined but presumed to be near junction of the common FGF R1 NH2-terminus and NH2-terminus of Ig loop II.
Localization
Cell Membrane
Specificity
Reacts with beta isoform of FRFR1. Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculoviral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: under investigation.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FGFR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Flow Cytometry 1:500-1:1000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Publications
Read Publication using NB100-2079.

Reactivity Notes

Human, mouse and rat. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FGF R1 Antibody (M17A3)

  • basic fibroblast growth factor receptor 1
  • BFGFR
  • bFGF-R-1
  • CD331 antigen
  • CD331
  • CEK
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R1
  • FGFBR
  • FGFR1
  • FGFR-1
  • fibroblast growth factor receptor 1
  • FLGH3
  • FLJ99988
  • Flt-2
  • FLT2H4
  • Fms-like tyrosine kinase 2
  • fms-related tyrosine kinase 2
  • H2
  • H5
  • HBGFR
  • heparin-binding growth factor receptor
  • hydroxyaryl-protein kinase
  • KAL 2
  • KAL2
  • N-SAM
  • OGD
  • Proto-oncogene c-Fgr
  • soluble FGFR1 variant 1
  • soluble FGFR1 variant 2

Background

FGF R1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGF R1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGF R1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGF R1 result in Pfeiffer syndrome associated with craniosynostosis. FGF R1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGF R1 has been shown to interact with N-cadherin and NCAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-00932
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
232-FA/CF
Species: Hu
MAB766
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF2265
Species: Mu
Applications: WB, IHC
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP1-76580
Species: Hu
Applications: WB, ELISA, ICC/IF, ICC
AF2180
Species: Hu
Applications: WB, Simple Western, IP
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF385
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut

Publications for FGF R1 Antibody (NB100-2079)(1)

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
All Species

Reviews for FGF R1 Antibody (NB100-2079) (0)

There are no reviews for FGF R1 Antibody (NB100-2079). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for FGF R1 Antibody (NB100-2079): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FGF R1 Antibody (NB100-2079). (Showing 1 - 3 of 3 FAQ).

  1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
    • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
  2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
    • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.
  3. Do you have any information on these questions regarding the flow picture on the product's datasheet? 1) Is the epitope extracellular? 2) Were the cells permeabilized before staining? 3) Is there any literature about this antibody and flow cytometry?
    • In answer to your questions: 1) The epitope is undetermined but presumed to be near junction of the common FGFr1 NH2-terminus and NH2-terminus of Ig loop II. 2) The immunogen was made to the extracellular domain, so permeabilization was not performed. 3) We unfortunately do not have any publications with this particular antibody.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2079AF405
Alexa Fluor 488 Labeled NB100-2079AF488
Alexa Fluor 647 Labeled NB100-2079AF647
Alexa Fluor 700 Labeled NB100-2079AF700
Allophycocyanin Labeled NB100-2079APC
Biotin Labeled NB100-2079B
DyLight 350 Labeled NB100-2079UV
DyLight 405 Labeled NB100-2079V
DyLight 488 Labeled NB100-2079G
DyLight 550 Labeled NB100-2079R
DyLight 650 Labeled NB100-2079C
DyLight 680 Labeled NB100-2079FR
DyLight 755 Labeled NB100-2079IR
HRP Labeled NB100-2079H
PE Labeled NB100-2079PE
PerCP Labeled NB100-2079PCP

Additional FGF R1 Products

Bioinformatics Tool for FGF R1 Antibody (NB100-2079)

Discover related pathways, diseases and genes to FGF R1 Antibody (NB100-2079). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FGF R1 Antibody (NB100-2079)

Discover more about diseases related to FGF R1 Antibody (NB100-2079).
 

Pathways for FGF R1 Antibody (NB100-2079)

View related products by pathway.

PTMs for FGF R1 Antibody (NB100-2079)

Learn more about PTMs related to FGF R1 Antibody (NB100-2079).
 

Research Areas for FGF R1 Antibody (NB100-2079)

Find related products by research area.

Blogs on FGF R1

There are no specific blogs for FGF R1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FGF R1 Antibody (M17A3) and receive a gift card or discount.

Bioinformatics

Gene Symbol FGFR1
Entrez
Uniprot