Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml ...read more
Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat ...read more
Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat ...read more
Recombinant human ectodomain of FGF R1 expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope is undetermined but presumed to be near junction of the common FGF R1 NH2-terminus and NH2-terminus of Ig loop II.
Reacts with beta isoform of FRFR1. Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculoviral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: under investigation.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Human, mouse and rat. Not yet tested in other species.
Alternate Names for FGF R1 Antibody (M17A3)
basic fibroblast growth factor receptor 1
fibroblast growth factor receptor 1
Fms-like tyrosine kinase 2
fms-related tyrosine kinase 2
heparin-binding growth factor receptor
soluble FGFR1 variant 1
soluble FGFR1 variant 2
FGF R1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGF R1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGF R1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGF R1 result in Pfeiffer syndrome associated with craniosynostosis. FGF R1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGF R1 has been shown to interact with N-cadherin and NCAM.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for FGF R1 Antibody (NB100-2079). (Showing 1 - 3 of 3 FAQ).
I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.
Do you have any information on these questions regarding the flow picture on the product's datasheet? 1) Is the epitope extracellular? 2) Were the cells permeabilized before staining? 3) Is there any literature about this antibody and flow cytometry?
In answer to your questions: 1) The epitope is undetermined but presumed to be near junction of the common FGFr1 NH2-terminus and NH2-terminus of Ig loop II. 2) The immunogen was made to the extracellular domain, so permeabilization was not performed. 3) We unfortunately do not have any publications with this particular antibody.