Recombinant Human FGF basic/FGF2 GMP Protein

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SDS-Page: Recombinant Human FGF basic/FGF2 Protein [NBP2-61389]

Product Details

Summary
Product Discontinued
View other related FGF basic/FGF2 Peptides and Proteins

Order Details


    • Catalog Number
      NBP2-61389
    • Availability
      Product Discontinued

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Recombinant Human FGF basic/FGF2 GMP Protein Summary

Description
A single non-glycosylated polypeptide chain containing 147 amino acids.

Source: Escherichia coli.

Amino Acid Sequence:
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Details of Functionality
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 107 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FGF2
Purity
>98%, by HPLC
Endotoxin Note
Less than 0.01 EU/ug of rHubFGF GMP as determined by LAL method.

Applications/Dilutions

Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
>98%, by HPLC
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Alternate Names for Recombinant Human FGF basic/FGF2 GMP Protein

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for FGF basic/FGF2 Recombinant Protein (NBP2-61389). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Bioinformatics

Gene Symbol FGF2