FGF basic/FGF2 Biologically Active Protein



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FGF basic/FGF2 Biologically Active Protein Summary

A biologically active protein corresponding to FGF2.
Produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Details of Functionality
Bioactivity of NovActive recombinant human FGF-basic 146 is assayed by measuring its dose dependent effect on proliferation of 3T3 cells. The ED50 for this effect using FGFb 146 is typically < 0.2 ng/ml, corresponding to a specific activity > 5 x10e6 U/mg.
Endotoxin Note
Endotoxin level is less than 0.005ng per ug (0.05EU/ug) as measured by turbidimetric kinetic assay

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Sterile filtered through a 0.2 um filter.PBS, pH 7.2.
No Preservative
Reconstitution Instructions
Reconstitute with deionized water.

Reactivity Notes

Human Recombinant protein


FGF basic from Novus Biologicals is now available for stem cell research. Novus uses a revolutionary technology to produce growth factors and cytokines to the highest purity. Furthermore our unique technology enables us to offer high quality FGF basic at lower prices than the market leaders. Purity is greater than 95% by SDS-PAGE gel analysis.


Functional: FGF-2 Protein [NBP2-13181] - We have tested the recombinant FGFb NBP2-13181 and found it to be very active and exactly as good as R&D Systems' FGFb in our bioassay.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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FAQs for FGF basic/FGF2 Protein (NBP2-13181). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Contact Information

Product PDFs


Gene Symbol FGF2

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