Recombinant Rat FGF basic/FGF2 Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications PAGE, HPLC
Concentration
Lyoph

Order Details

Recombinant Rat FGF basic/FGF2 Protein Summary

Description
A recombinant protein corresponding to 145 amino acids of Rat FGF basic/FGF2
Preparation
Method
>97% SDS-PAGE
Details of Functionality
The ED50 determined by the dose-dependent proliferation of BALB/3T3 cells is <0.2 ng/ml, corresponding to a specific activity of 5x106 IU/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
FGF2
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Theoretical MW
16.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from solution after extensive dialysis against 20 mM phosphate buffer, pH 7.4 and 130 mM NaCl.
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute in sterile H2O to 0.1-1 mg /ml. The solution can then further diluted to other aqueous solutions

Alternate Names for Recombinant Rat FGF basic/FGF2 Protein

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Background

FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. The recombinant rat FGF-2 is a single, non-glycosylated chain containing 145 amino acids and having a molecular weight of 16.3 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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FAQs for FGF basic/FGF2 Protein (NBP1-99874). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Bioinformatics

Gene Symbol FGF2
Entrez