Porcine FGF basic/FGF2 ELISA Kit (Colorimetric)

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Porcine FGF basic/FGF2 ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

Porcine FGF basic/FGF2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Porcine bFGF/FGF2 in samples. No significant cross-reactivity or interference between Porcine bFGF/FGF2 and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.24%
Intra-Assay
5.10%
Spike Recovery
83-101%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FGF2

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Certificate of Analysis
  2. Biotinylated Detection Ab Diluent
  3. Concentrated Wash Buffer (25×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Biotinylated Detection Ab (100×)
  6. Micro ELISA Plate(Dismountable)
  7. HRP Conjugate Diluent
  8. Plate Sealer
  9. Product Description
  10. Reference Standard
  11. Reference Standard & Sample Diluent
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Porcine FGF basic/FGF2 ELISA Kit (Colorimetric)

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for FGF basic/FGF2 ELISA Kit (NBP2-69961). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

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Bioinformatics

Gene Symbol FGF2