FGF acidic/FGF1 Recombinant Protein


SDS-Page: FGF1 Protein [NBC1-18465] - aFGF, 15.9kDa (141aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Reactivity HuSpecies Glossary
Applications PAGE
1.0 mg/ml

Order Details

FGF acidic/FGF1 Recombinant Protein Summary

A recombinant protein corresponding to FGF1.


Details of Functionality
The ED50 for this effect is < 2.9 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell (which corresponds to > 340,000 units/ml).
>95% pure by SDS-PAGE

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Liquid. 20 mM Tris, pH 7.0, 100 mM NaCl
No Preservative
1.0 mg/ml
>95% pure by SDS-PAGE


  • SDS-Page
Application Notes
1. Cell Line: NIH-3T3 (Mus mosculus/ embryo)
2. Maintenance Condition: RPMI 1640 containing 10% FBS
3. Assay Medium: serum free RPMI 1640
4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul)
5. Serum Free Starvation: 24hr with RPMI1640
6. Incubation Time: 24 hr (after sample treatment)
7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution)
8. Detection method: BrdU assay

Reactivity Notes



The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 15.9kDa (141aa), confirmed by MALDI-TOF.

Alternate Names for FGF acidic/FGF1 Recombinant Protein

  • AFGF
  • alpha
  • alpha-ECGF
  • beta-ECGF
  • ECGF
  • ECGF-betaAcidic fibroblast growth factor
  • endothelial cell growth factor, beta
  • FGF1
  • FGF-1
  • FGFABeta-endothelial cell growth factor
  • FGF-alpha
  • fibroblast growth factor 1 (acidic)
  • GLIO703
  • HBGF1
  • HBGF-1
  • heparin-binding growth factor 1


Acidic fibroblast growth factor(aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Contact Information

Product PDFs


Gene Symbol FGF1

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