Recombinant Human FGF-8 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
Lyoph

Order Details

Recombinant Human FGF-8 Protein Summary

Description
Recombinant bioactive protein containing 193 amino acids for Human FGF-8

Source:E. coli
Amino Acid Sequence:(P55075)

Details of Functionality
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.5 ng/ml, corresponding to a specific activity of >2 x 106 units/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
FGF8
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Theoretical MW
22.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized in 10 mM Tris, pH 8.0 + 75 mM NaCl. The protein may appear as a haze or film, which may not be seen at the bottom of the vial.
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in 5 mM Tris, pH 8.0 to a concentration of 0.1-1 mg/ml. This solution can be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use. Repeated freeze/thaw cycles will result in some loss of activity.

Alternate Names for Recombinant Human FGF-8 Protein

  • AIGF
  • AIGFKAL6
  • Androgen-induced growth factor
  • FGF8
  • FGF-8
  • fibroblast growth factor 8 (androgen-induced)
  • fibroblast growth factor 8
  • HBGF-8
  • Heparin-binding growth factor 8
  • MGC149376

Background

Fibroblast Growth Factor-8 (FGF-8) is also known as Androgen-induced Growth Factor (AIGF), a heparin binding growth factor, which stimulates the proliferation and activation of cells that express the FGF receptors. Recombinant human FGF-8 is a 22.4 kDa protein containing 193 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol FGF8
Entrez