Fer Knockout HeLa Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

Fer Knockout HeLa Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,-13 bp deletion in exon4
Gene
FER

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout HeLa Cell

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for Fer Knockout HeLa Cell Lysate

  • EC 2.7.10
  • EC 2.7.10.2
  • fer (fps/fes related) tyrosine kinase
  • Fer
  • p94-Fer
  • Phosphoprotein NCP94
  • Proto-oncogene c-Fer
  • TYK3
  • TYK3phosphoprotein NCP94
  • Tyrosine kinase 3
  • tyrosine-protein kinase Fer

Background

The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol FER
Entrez