FDFT1 Knockout 293T Cell Lysate

Images

 

Product Details

Summary
Product Discontinued
View other related FDFT1 Lysates

Order Details


    • Catalog Number
      NBP2-65728
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

FDFT1 Knockout 293T Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,Insertion of the selection cassette in exon 1
Gene
FDFT1

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout 293T Cell
Life Stage
Embryonic
Tissue
Kidney

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for FDFT1 Knockout 293T Cell Lysate

  • DGPT
  • EC 2.5.1.21
  • ERG9
  • farnesyl-diphosphate farnesyltransferase 1
  • Farnesyl-diphosphate farnesyltransferase
  • FPP:FPP farnesyltransferase
  • presqualene-di-diphosphate synthase
  • SQS
  • squalene synthase
  • squalene synthetase
  • SS

Background

This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-16463
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-66888
Species: Hu, Mu, Rt
Applications: WB, ChIP, IP
NBP2-32001
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-83368
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-85445
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
MAB1228
Species: Hu
Applications: Flow, IHC, IP, CyTOF-ready
NB100-417
Species: Hu, Mu, Rt, Pl
Applications: WB, Simple Western, ChIP, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, RIA
NB600-582
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha, Mk
Applications: WB, Simple Western, IHC
NBP1-54446
Species: Hu, Ch
Applications: WB, ELISA, GS, ICC/IF, IP
AF8516
Species: Hu
Applications: WB, Simple Western, IP, ICC
NBP1-53166
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ze
Applications: WB, IHC
AF5535
Species: Hu
Applications: WB, Simple Western
NB600-233
Species: Hu, Mu
Applications: WB, ChIP, IHC, IHC-P, IP, CHIP-SEQ
NB200-540
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC

Publications for FDFT1 Lysate (NBP2-65728) (0)

There are no publications for FDFT1 Lysate (NBP2-65728).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FDFT1 Lysate (NBP2-65728) (0)

There are no reviews for FDFT1 Lysate (NBP2-65728). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FDFT1 Lysate (NBP2-65728) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional FDFT1 Products

Bioinformatics Tool for FDFT1 Lysate (NBP2-65728)

Discover related pathways, diseases and genes to FDFT1 Lysate (NBP2-65728). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FDFT1 Lysate (NBP2-65728)

Discover more about diseases related to FDFT1 Lysate (NBP2-65728).
 

Pathways for FDFT1 Lysate (NBP2-65728)

View related products by pathway.

PTMs for FDFT1 Lysate (NBP2-65728)

Learn more about PTMs related to FDFT1 Lysate (NBP2-65728).

Blogs on FDFT1

There are no specific blogs for FDFT1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FDFT1 Knockout 293T Cell Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol FDFT1