FBXO21 Antibody (2C7)

Images

 
Western Blot: FBXO21 Antibody (2C7) [NBP2-01569] Analysis of extracts (35ug) from 9 different cell lines by using anti-FBXO21 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: FBXO21 Antibody (2C7) [NBP2-01569] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FBXO21.
Immunohistochemistry-Paraffin: FBXO21 Antibody (2C7) [NBP2-01569] - Staining of paraffin-embedded Human pancreas tissue using anti-FBXO21 mouse monoclonal antibody.
Flow Cytometry: FBXO21 Antibody (2C7) [NBP2-01569] - Analysis of Jurkat cells, using anti-FBXO21 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: FBXO21 Antibody (2C7) [NBP2-01569] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO21 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: FBXO21 Antibody (2C7) [NBP2-01569] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FBXO21 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FBXO21 Antibody (2C7) [NBP2-01569] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FBXO21 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FBXO21 Antibody (2C7) [NBP2-01569] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FBXO21 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FBXO21 Antibody (2C7) [NBP2-01569] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FBXO21 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: FBXO21 Antibody (2C7) [NBP2-01569] - Staining of paraffin-embedded Human Kidney tissue using anti-FBXO21 mouse monoclonal antibody.
Flow Cytometry: FBXO21 Antibody (2C7) [NBP2-01569] - Analysis of Hela cells, using anti-FBXO21 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

FBXO21 Antibody (2C7) Summary

Immunogen
Full length human recombinant protein of human FBXO21(NP_296373) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FBXO21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human, Dog, Rat, Monkey, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FBXO21 Antibody (2C7)

  • DKFZp434G058
  • F-box only protein 21
  • F-box protein 21
  • FBX21FLJ90233
  • KIAA0875MGC26682

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FBXO21 Antibody (NBP2-01569) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FBXO21
Entrez