Fas/TNFRSF6/CD95 Antibody

Images

 
Western Blot: Fas/TNFRSF6/CD95 Antibody [NB100-92093] - Analysis of CD95 (L315) pAb at 1:500 dilutionLane1:Hela whole cell lysate(40ug) Lane2:RAW264.7 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug)
Immunohistochemistry-Paraffin: Fas/TNFRSF6/CD95 Antibody [NB100-92093] - Analyzes of CD95 (L315) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: Fas/TNFRSF6/CD95 Antibody [NB100-92093] - Analysis of FAS (L315) antibody in extracts from LOVO cells.
Western Blot: Fas/TNFRSF6/CD95 Antibody [NB100-92093] - Analysis of CD95 (L315) pAb at 1:500 dilutionLane1:Hela cell lysateLane2:H9C2 cell lysateLane3:Mouse brain tissue lysateLane4:Rat brain tissue lysate
Immunohistochemistry-Paraffin: Fas/TNFRSF6/CD95 Antibody [NB100-92093] - Paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92093
    • Availability
      Product Discontinued

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Fas/TNFRSF6/CD95 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 280-330 of Human CD95.
Clonality
Polyclonal
Host
Rabbit
Gene
FAS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of FAS (L315) pAb in extracts from LOVO cells.
Immunohistochemistry (IHC) analyzes of FAS (L315) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Fas/TNFRSF6/CD95 Lysate (NBL1-10598)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fas/TNFRSF6/CD95 Antibody

  • Apo-1 antigen
  • Apo-1
  • apoptosis antigen 1
  • Apoptosis-mediating surface antigen FAS
  • APT1
  • APT1FASTM
  • CD95 antigen
  • CD95
  • CD95ALPS1A
  • Fas (TNF receptor superfamily, member 6)
  • Fas AMA
  • Fas antigen
  • Fas
  • FAS1
  • FASLG receptor
  • TNFRSF6
  • TNFRSF6member 6
  • tumor necrosis factor receptor superfamily member 6

Background

Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immuno- surveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FASbased mechanism involves the transducing molecule FAS (also designated APO-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS. These findings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FAS may be critically involved in CTL-mediated cytotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Fas/TNFRSF6/CD95 Antibody (NB100-92093)

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Bioinformatics

Gene Symbol FAS
Entrez