Fas Ligand/TNFSF6 Antibody (Kay-10)

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Flow Cytometry: Fas Ligand/TNFSF6 Antibody (Kay-10) [NB100-77497] - Analysis using the Biotin conjugate of NB100-77497. Staining of Mouse Fas Ligand transfected cells with biotinylated KAY-10, followed by Sav-PE.
Flow Cytometry: Fas Ligand/TNFSF6 Antibody (Kay-10) [NB100-77497] - Mouse Fas Ligand transfected cells stained with purified KAY-10, followed by biotinylated anti-mouse IgG and Sav-PE

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-77497
    • Availability
      Product Discontinued

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Fas Ligand/TNFSF6 Antibody (Kay-10) Summary

Immunogen
C57BL/6 mouse FasL cDNA-transfected L5178Y cells
Specificity
CD178.1 (Kay-10)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FASLG
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry-paraffin. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Fas Ligand/TNFSF6 Antibody (Kay-10)

  • apoptosis (APO-1) antigen ligand 1
  • Apoptosis antigen ligand
  • APT1LG1CD95L
  • APTL
  • CD178 antigen
  • CD178
  • CD95L
  • CD95-L
  • Fas antigen ligand
  • Fas ligand (TNF superfamily, member 6)
  • Fas Ligand
  • FASLCD95 ligand
  • FASLG
  • TNFSF6
  • TNFSF6FasL
  • tumor necrosis factor (ligand) superfamily, member 6
  • tumor necrosis factor ligand superfamily member 6

Background

CD178.1 is a 40 kD Fas ligand alloantigen (designated as mFasL.1) expressed on the activated T lymphocytes, testis, and eye of selected mouse strains including C57BL/6, C3H, MRL, NOD, NZB, NZW, and SJL. It does not react with lymphoblasts from BALB/c, DBA/1, or DBA/2 mice. CD178.1 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. Functional studies suggest that mFasL.2 has higher specific activity than mFasL.1. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The Kay-10 antibody has been reported to block CD178.1/CD95 induced apoptosis in cells derived from mice expressing this alloantigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FASLG
Entrez