FANCD2 Antibody

Images

 
Western Blot: FANCD2 Antibody [NB100-91762] - FANCD2 antibody (P216) in extracts from HT-29 treated with Calyculin A 50ng/ml 30'.
Immunohistochemistry-Paraffin: FANCD2 Antibody [NB100-91762] - Paraffin-embedded human breast carcinoma tissue.
Immunohistochemistry: FANCD2 Antibody [NB100-91762] - Paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related FANCD2 Primary Antibodies

Order Details


    • Catalog Number
      NB100-91762
    • Availability
      Product Discontinued

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FANCD2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 182-234 of Human FANCD2.
Clonality
Polyclonal
Host
Rabbit
Gene
FANCD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of FANCD2 (P216) pAb in extracts from HT-29 treated with Calyculin A 50ng/ml 30'.
Immunohistochemistry (IHC) analyzes of FANCD2 (P216) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
166 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FANCD2 Antibody

  • DKFZp762A223
  • FA4
  • FACD
  • FACDFLJ23826
  • FAD
  • FAD2
  • FA-D2
  • FADFAD2
  • FANCD
  • FANCD2
  • Fanconi anemia group D2 protein
  • Fanconi anemia, complementation group D2
  • Protein FACD2

Background

Fanconi anemia (FA) is an autosomal recessive disorder characterized by bone marrow failure, birth defects and chromosomal instability. At the cellular level, FA is characterized by spontaneous chromosomal breakage and a unique hypersensitivity to DNA cross-linking agents. At least 8 complementation groups (A-G) have been identified and 6 FA genes (for subtypes A, C, D2, E, F and G) have been cloned. The FA proteins lack sequence homologies or motifs that could point to a molecular function. Phosphorylation of FANC (Fanconi anemia complementation group) proteins are thought to be important for the function of the FA pathway. Several FA proteins, including FANCA, FANCC, FANCF, and FANCG, interact in a nuclear complex, and this complex is required for the activation (monoubiquitination) of the downstream FANCD2 protein. When monoubiquitinated, the FANCD2 protein co-localizes with the breast cancer susceptibility protein BRCA1 in DNA damage induced foci. In male meiosis, FANCD2 also co-localizes with BRCA1 at synaptonemal complexes. The human FANCD2 gene maps to chromosome 3p25.3, contains 44 exons and encodes a 1,451 amino acid nuclear protein that exists as two protein isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional FANCD2 Products

FANCD2 NB100-91762

Bioinformatics Tool for FANCD2 Antibody (NB100-91762)

Discover related pathways, diseases and genes to FANCD2 Antibody (NB100-91762). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FANCD2 Antibody (NB100-91762)

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Pathways for FANCD2 Antibody (NB100-91762)

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PTMs for FANCD2 Antibody (NB100-91762)

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Research Areas for FANCD2 Antibody (NB100-91762)

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Blogs on FANCD2.

FANCD2 and DNA damage repair
Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t...  Read full blog post.

FANCD2 (Fanconi anemia subunit D2 protein)
Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama...  Read full blog post.

FANCD2: A big component of the DNA repair crew
The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage...  Read full blog post.

FANCD2: DNA Repair and Beyond
Fanconi anemia (FANC) is a heterogenous, autosomal-recessive cancer susceptibility genetic disorder that is characterized by a wide array of symptoms, including congenital defects, progressive bone marrow failure due to DNA-damage hypersensitivity, ch...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Fanconi Antibodies and Cancer Research
We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)...  Read full blog post.

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Bioinformatics

Gene Symbol FANCD2
Entrez