FAK Antibody

Images

 
Western Blot: FAK Antibody [NBP2-16408] - Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: FAK Antibody [NBP2-16408] - Immunohistochemical analysis of paraffin-embedded cal27 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

FAK Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human FAK. The exact sequence is proprietary.
Localization
Cell junction; focal adhesion; Cell membrane; Peripheral membrane protein; Cytoplasmic side
Marker
Focal Adhesion Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FAK Lysate (NBP2-65784)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for FAK Antibody

  • EC 2.7.10
  • FADK 1
  • FADK1
  • FADKFAK-related non-kinase polypeptide
  • FAK
  • FAK1FRNK
  • FAKEC 2.7.10.2
  • focal adhesion kinase 1
  • pp125FAK
  • Protein-tyrosine kinase 2
  • PTK2 protein tyrosine kinase 2
  • PTK2

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTK2
Entrez
Uniprot