FADD Antibody

Product Discontinued

FADD Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human FADD.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FADD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of FADD (N188) pAb in extracts from HeLa cells treated with PMA 125ng/ml 30'.
  Immunohistochemistry (IHC) analyzes of FADD (N188) pAb in paraffin-embedded human lung carcinoma tissue. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26972597).
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FADD Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FADD Antibody

• FADD
• Fas (TNFRSF6)-associated via death domain
• FAS-associated death domain protein
• FAS-associating death domain-containing protein
• GIG3
• Growth-inhibiting gene 3 protein
• Mediator of receptor induced toxicity
• mediator of receptor-induced toxicity
• MGC8528
• MORT1
• MORT1Fas-associating protein with death domain
• protein FADD

Background

Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1, and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1, which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8, which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also, the deficiency in FADD can inhibit TRAIL-induced cell apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FADD Antibody (NB100-92032)(2)

Publications using NB100-92032

• Ranjan K, Pathak C. FADD regulates NF-kB activation and promotes ubiquitination of cFLIPL to induce apoptosis. Sci Rep. 2016-03-14 [PMID: 26972597] (WB, ICC/IF, Human)
• Ranjan K, Surolia A, Pathak C. Apoptotic potential of Fas-associated death domain on regulation of cell death regulatory protein cFLIP and death receptor mediated apoptosis in HEK 293T cells. J Cell Commun Signal. 2012-08-01 [PMID: 22791313] (WB, Human)

Review for FADD Antibody (NB100-92032) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Kishu Ranjan WB Human 01/31/2014

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: FADD
• Entrez:
  • 8772(Human)