F-box protein 43 Antibody

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Western Blot: F-box protein 43 Antibody [NB600-890] - Analysis. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB600-890
    • Availability
      Product Discontinued

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F-box protein 43 Antibody Summary

Immunogen
Synthetic peptide: SPPIVNSKYSTFRDFC, corresponding to amino acids 53 - 68 of Human Fbp5B.
Specificity
This recognises an in vitro translation of Fbp5b. It also recognises a single slightly larger band in 293T cells that is reduced by peptide blocking. We believe this is strong evidence that the is specifically recognising Fbp5b.
Clonality
Polyclonal
Host
Rabbit
Gene
FBXO43
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:10000-1:50000
  • Immunoprecipitation 1:100
Application Notes
This affinity-purified antibody has been tested for use in ELISA and western blot. Expect a band approximately 105 kDa in size corresponding - Fbp5B protein by western blotting in the appropriate cell lysate or extract. Predicted molecular weight is 51 kDa.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.

Alternate Names for F-box protein 43 Antibody

  • early mitotic inhibitor 2
  • EMI2Fbx43
  • Endogenous meiotic inhibitor 2
  • ERP1
  • F-box only protein 43
  • F-box protein 43
  • FBX43

Background

This antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa corresponding to CDK9 (PITALRE) by western blotting in the appropriate cell lysate or extract.Fbp5B (also called F-Box protein 43 or Fbx043) is a relatively new member of the Fbox protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different proteinprotein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FBXO43