F-Actin Antibody (NH3)

Images

 
Immunocytochemistry/ Immunofluorescence: F-Actin Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
Clone
NH3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

F-Actin Antibody (NH3) Summary

Immunogen
Human monocytes and U937 cell line
Specificity
Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
ACTG1
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • ELISA 1:10
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
ICC data from customer review. Staining on paraffin section negative as per customer feedback.
Reviewed Applications
Read 1 Review rated 5
using
NB100-64792 in the following applications:

Publications
Read Publications using
NB100-64792 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for F-Actin Antibody (NH3)

  • actin, cytoplasmic 2
  • DFNA26
  • ACT
  • ACTG
  • actin, gamma 1
  • BRWS2
  • DFNA20

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-64792
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr

Publications for F-Actin Antibody (NB100-64792)(3)

Review for F-Actin Antibody (NB100-64792) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-64792:
Filter by Applications
IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence F-Actin NB100-64792
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reviewed by:
Chan-Yen Kuo
IF Human 07/05/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
Pub Med IDdoi: 10.1016/j.virusres.2013.03.017. Epub 2013 Apr 13
FileView PDF

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for F-Actin Antibody (NB100-64792) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64792AF405
Alexa Fluor 488 Labeled NB100-64792AF488
Alexa Fluor 647 Labeled NB100-64792AF647
Alexa Fluor 700 Labeled NB100-64792AF700
Allophycocyanin Labeled NB100-64792APC
DyLight 350 Labeled NB100-64792UV
DyLight 405 Labeled NB100-64792V
DyLight 488 Labeled NB100-64792G
DyLight 680 Labeled NB100-64792FR
DyLight 755 Labeled NB100-64792IR
PE Labeled NB100-64792PE
PerCP Labeled NB100-64792PCP

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F-Actin NB100-64792

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Blogs on F-Actin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Chan-Yen Kuo
07/05/2013
Application: IF
Species: Human

Bioinformatics

Gene Symbol ACTG1
Entrez
Uniprot