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F-Actin Antibody (NH3)

 (3 Publications)
 (1 Review)
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Immunocytochemistry/Immunofluorescence: F-Actin Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: F-Actin Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
Clone
NH3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-64792AF405
Alexa Fluor 488
NB100-64792AF488
Alexa Fluor 647
NB100-64792AF647
Alexa Fluor 700
NB100-64792AF700
Allophycocyanin
NB100-64792APC
DyLight 350
NB100-64792UV
DyLight 405
NB100-64792V
DyLight 488
NB100-64792G
DyLight 680
NB100-64792FR
DyLight 755
NB100-64792IR
PE
NB100-64792PE
PerCP
NB100-64792PCP
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F-Actin Antibody (NH3) Summary

Immunogen
Human monocytes and U937 cell line
Specificity
Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
ACTG1
Purity
IgM purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • ELISA 1:10
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
ICC data from customer review. Staining on paraffin section negative as per customer feedback.
Reviewed Applications
Read 1 Review rated 5
using
NB100-64792 in the following applications:
Publications
Read Publications using
NB100-64792 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for F-Actin Antibody (NH3)

  • actin, cytoplasmic 2
  • DFNA26
  • ACT
  • ACTG
  • actin, gamma 1
  • BRWS2
  • DFNA20

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH, NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
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Immunohistochemistry-Paraffin: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Paraffin-embedded rat pancreas. Carboxypeptidase B antibody [N3C3] diluted at 1:1000.
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP2-15698] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody [N3C3] diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.
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Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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NBP1-33527
Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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F-Actin Antibody
(NH3)
NB100-64792
F-Actin Antibody (NH3)
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr

Publications for F-Actin Antibody (NB100-64792)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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Review for F-Actin Antibody (NB100-64792) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-64792:
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Images Ratings Applications Species Date Details
Immunofluorescence F-Actin NB100-64792
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reviewed by:
Chan-Yen Kuo
 IF Human 07/05/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
Pub Med IDdoi: 10.1016/j.virusres.2013.03.017. Epub 2013 Apr 13
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FAQs for F-Actin Antibody (NB100-64792) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64792AF405
Alexa Fluor 488 Labeled NB100-64792AF488
Alexa Fluor 647 Labeled NB100-64792AF647
Alexa Fluor 700 Labeled NB100-64792AF700
Allophycocyanin Labeled NB100-64792APC
DyLight 350 Labeled NB100-64792UV
DyLight 405 Labeled NB100-64792V
DyLight 488 Labeled NB100-64792G
DyLight 680 Labeled NB100-64792FR
DyLight 755 Labeled NB100-64792IR
PE Labeled NB100-64792PE
PerCP Labeled NB100-64792PCP
Learn about Custom Antibody Labeling

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Blogs on F-Actin.

Check out the latest blog posts on F-Actin.
The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

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Immunofluorescence F-Actin NB100-64792
Enlarge
 Chan-Yen Kuo
07/05/2013
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol ACTG1
Entrez
Uniprot