F-Actin Antibody (NH3)

Immunocytochemistry/Immunofluorescence: F-Actin Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
• Clone: NH3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

F-Actin Antibody (NH3) Summary

• Immunogen: Human monocytes and U937 cell line
• Specificity: Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTG1
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:500
  • ELISA 1:10
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: ICC data from customer review. Staining on paraffin section negative as per customer feedback.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: IgM purified

Alternate Names for F-Actin Antibody (NH3)

• actin, cytoplasmic 2
• DFNA26
• ACT
• ACTG
• actin, gamma 1
• BRWS2
• DFNA20

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for F-Actin Antibody (NB100-64792)(3)

Publications using NB100-64792

• Liu R, Lauridsen HM, Amezquita RA et al. IL-17 Promotes Neutrophil-Mediated Immunity by Activating Microvascular Pericytes and Not Endothelium. J. Immunol. Aug 17 2016 [PMID: 27534549] (ICC/IF, Human)
• Goncalves NP, Martins D, Saraiva MJ. Overexpression of Protocadherin-10 in Transthyretin-Related Familial Amyloidotic Polyneuropathy. Am. J. Pathol. Jul 1 2016 [PMID: 27338109]
• Cheng WY, Chiao MT, Liang YJ et al. Luteolin inhibits migration of human glioblastoma U-87 MG and T98G cells through downregulation of Cdc42 expression and PI3K/AKT activity. Mol Biol Rep 2013 May 16 [PMID: 23677714] (WB, Human)

Review for F-Actin Antibody (NB100-64792) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Chan-Yen Kuo IF Human 07/05/2013

Summary

• Application: Immunofluorescence
• Species: Human
• Pub Med ID: doi: 10.1016/j.virusres.2013.03.017. Epub 2013 Apr 13
• File: View PDF

Bioinformatics

• Gene Symbol: ACTG1
• Entrez:
  • 71(Human)
• Uniprot:
  • P63261(Human)