EZH2/KMT6 RNAi

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications RNAi, RNAi SP

Order Details

EZH2/KMT6 RNAi Summary

Specificity
enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, mRNA
Gene
EZH2

Applications/Dilutions

Application Notes
This RNAi causes protein knockdown.
Reviewed Applications
Read 1 Review rated 4
using
H00002146-R01 in the following application:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EZH2/KMT6 RNAi

  • EC 2.1.1
  • EC 2.1.1.43
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste 2
  • enhancer of zeste homolog 2 (Drosophila)
  • Enhancer of zeste homolog 2
  • ENX1
  • ENX-1KMT6EZH1KMT6A
  • EZH2
  • histone-lysine N-methyltransferase EZH2
  • KMT6
  • Lysine N-methyltransferase 6
  • MGC9169

Background

Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene.  The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo.  Chimera RNAi has many advantages over the conventional siRNAs.  First, it has been demonstrated to have reliable knock-down for over 10,000 human genes.  Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence.  Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected.  Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. RNAi are guaranteed for 3 months from date of receipt.

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Publications for KMT6/EZH2 RNAi (H00002146-R01) (0)

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Review for KMT6/EZH2 RNAi (H00002146-R01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00002146-R01:
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WB
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Western Blot EZH2/KMT6 H00002146-R01
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reviewed by:
Alex Schmidt
WB Human 11/25/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa cells
SpeciesHuman

Blocking

Blocking Details4 % BSA in TBST

Primary Anitbody

Dilution RatioDiluted 1:1000 times

Secondary Antibody

Secondary DescriptionGoat Anti-Mouse IgG HRP Affinity Purified PAb (R&D Systems)
Secondary Manufacturer Cat#HAF007
Secondary ConcentrationDilution: 5,000 times

Details

Detection NotesOne-hour Western detection system (Genscript), 30 sec exposure

Comments

CommentsI could achieve 70-80% knockdown using this siRNA.

FAQs for KMT6/EZH2 RNAi (H00002146-R01) (0)

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1
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Alex Schmidt
11/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol EZH2
Entrez