Eukaryotic translation initiation factor 5B Antibody

Images

 
Western Blot: Eukaryotic translation initiation factor 5B Antibody [NBP1-30511] - Analysis of eIF5B (H1086) pAb in extracts from Jurkat cells.
Immunohistochemistry-Paraffin: Eukaryotic translation initiation factor 5B Antibody [NBP1-30511] - Analyzes of eIF5B (H1086) pAb in paraffin-embedded human breast carcinoma tissue.

Order Details


    • Catalog Number
      NBP1-30511
    • Availability
      Product Discontinued

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Eukaryotic translation initiation factor 5B Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 1061-1110 of Human eIF5B.
Specificity
eIF5B (H1086) antibody detects endogenous levels of eIF5B protein.
Clonality
Polyclonal
Host
Rabbit
Gene
EIF5B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of eIF5B (H1086) pAb in extracts from Jurkat cells.
Immunohistochemistry (IHC) analyzes of eIF5B (H1086) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human,Mouse,Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Eukaryotic translation initiation factor 5B Antibody

  • DKFZp434I036
  • eukaryotic translation initiation factor 5B
  • FLJ10524
  • IF2Translation initiation factor IF-2
  • KIAA0741eIF-5B
  • translation initiation factor IF2

Background

Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EIF5B