ETFA Antibody

Images

 
Western Blot: ETFA Antibody [NBP1-54677] - Human Hela, Antibody Dilution: 1.0 ug/ml ETFA is supported by BioGPS gene expression data to be expressed in HeLa.
Immunohistochemistry: ETFA Antibody [NBP1-54677] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.
Western Blot: ETFA Antibody [NBP1-54677] - Human Heart lysate, concentration 0.2-1 ug/ml.
Western Blot: ETFA Antibody [NBP1-54677] - Human HepG2, Antibody Dilution: 1.0 ug/ml ETFA is supported by BioGPS gene expression data to be expressed in HepG2.

Product Details

Summary
Product Discontinued
View other related ETFA Primary Antibodies

Order Details


    • Catalog Number
      NBP1-54677
    • Availability
      Product Discontinued

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ETFA Antibody Summary

Immunogen
Synthetic peptides corresponding to ETFA(electron-transfer-flavoprotein, alpha polypeptide (glutaric aciduria II)) The peptide sequence was selected from the N terminal of ETFA. Peptide sequence FRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAAT
Specificity
This product is specific to Subunit or Isoform: alpha, mitochondrial.
Clonality
Polyclonal
Host
Rabbit
Gene
ETFA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry
Application Notes
This is a rabbit polyclonal antibody against ETFA and was validated on Western blot.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ETFA Lysate (NBP2-65815)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for ETFA Antibody

  • alpha-ETF
  • electron transfer flavoprotein alpha-subunit
  • Electron transfer flavoprotein subunit alpha, mitochondrial
  • electron-transfer-flavoprotein, alpha polypeptide
  • EMA
  • GA2
  • glutaric aciduria II
  • MADD

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ETFA
Uniprot