Estrogen Receptor Antibody (1D5)

Images

 
Formalin fixed paraffin embedded human breast carcinoma stained with Estrogen Receptor antibody.

Product Details

Summary
Product Discontinued
View other related Estrogen Receptor Primary Antibodies

Order Details


    • Catalog Number
      NB120-17087
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Estrogen Receptor Antibody (1D5) Summary

Immunogen
Recombinant full length protein (Human).
Localization
Nuclear
Specificity
This antibody is specific to the estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. Nuclei of estrogen receptor positive cells stain very strongly with the antibody, without staining in the cytoplasm. On cryostat sections, positive staining of the estrogen receptor in the nucleus and cytoplasm can be seen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ESR1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
IHC-P: 1/25 - 1/50. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostainingWB: 1/50 - 1/100. Predicted molecular weight: 66 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
MCF-7 Lysate (NB820-59465)

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
Sodium Azide
Purity
Protein A or G purified

Alternate Names for Estrogen Receptor Antibody (1D5)

  • ER
  • Era
  • ER-alpha
  • ESRESRA
  • Estradiol receptor
  • estrogen receptor 1
  • estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
  • estrogen receptor alpha delta 4 +49 isoform
  • estrogen receptor alpha delta 4*5,6,7*/654 isoform
  • estrogen receptor
  • NR3A1DKFZp686N23123
  • Nuclear receptor subfamily 3 group A member 1

Background

This antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. However, on cryostat sections a positive staining of estrogen receptor in the nucleus as well as cytoplasm can be seen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5415
Species: Hu
Applications: WB, IHC, ICC
NB200-305
Species: Hu, Mu, Mk
Applications: WB, ChIP, DB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-07799
Species: Hu
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB100-1596
Species: Hu, Pm, Rb
Applications: WB, ICC/IF, IHC
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-89146
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-16468
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NB120-17087
Species: Hu
Applications: WB, IHC, IHC-P

Publications for Estrogen Receptor Antibody (NB120-17087) (0)

There are no publications for Estrogen Receptor Antibody (NB120-17087).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Estrogen Receptor Antibody (NB120-17087) (0)

There are no reviews for Estrogen Receptor Antibody (NB120-17087). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Estrogen Receptor Antibody (NB120-17087) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Estrogen Receptor Products

Estrogen Receptor NB120-17087

Bioinformatics Tool for Estrogen Receptor Antibody (NB120-17087)

Discover related pathways, diseases and genes to Estrogen Receptor Antibody (NB120-17087). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Estrogen Receptor Antibody (NB120-17087)

Discover more about diseases related to Estrogen Receptor Antibody (NB120-17087).
 

Pathways for Estrogen Receptor Antibody (NB120-17087)

View related products by pathway.

PTMs for Estrogen Receptor Antibody (NB120-17087)

Learn more about PTMs related to Estrogen Receptor Antibody (NB120-17087).
 

Research Areas for Estrogen Receptor Antibody (NB120-17087)

Find related products by research area.

Blogs on Estrogen Receptor.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

Androgen Receptor: What Makes a Man?
Steroid receptors (SRs) are a superfamily of ligand-dependent nuclear transcription factors that activate responsive genes response to hormone. Androgen receptors (ARs) are found in a wide variety of tissues, including reproductive organs, central ner...  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Estrogen Receptor Antibody (1D5) and receive a gift card or discount.

Bioinformatics

Gene Symbol ESR1
Entrez