Erythropoietin Antibody [Unconjugated]

Images

 
Erythropoietin was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Erythropoietin Polyclonal Antibody (Catalog # AB-286-NA) at 15 µg/mL for 1 hour at room ...read more
Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in theTF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Erythropoietin Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line (CHO)-derived recombinant human Erythropoietin
Specificity
Detects human Erythropoietin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPO
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization <3 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AB-286-NA in the following applications:

Publications
Read Publications using
AB-286-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin Antibody [Unconjugated]

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of Epo is required for biological activities in vivo. Mature human Epo shares 75%‑84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells. Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. Epo additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Erythropoietin Antibody (AB-286-NA)(2)

We have publications tested in 2 confirmed species: Human, Transgenic Hamster.

We have publications tested in 2 applications: ICC, Western Blot.


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ICC
(1)
Western Blot
(1)
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Human
(1)
Transgenic Hamster
(1)
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Review for Erythropoietin Antibody (AB-286-NA) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using AB-286-NA:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Other 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 19127370
SpeciesOther

Blocking

Blocking DetailsSee PMID 19127370

Details

Detection NotesSee PMID 19127370

Comments

CommentsSample Species: eucaryotic unicellular green alga Chlamydomonas reinhardtiiPMID 19127370

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FAQs for Erythropoietin Antibody (AB-286-NA) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Erythropoietin Antibody (AB-286-NA)

Discover related pathways, diseases and genes to Erythropoietin Antibody (AB-286-NA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Erythropoietin Antibody (AB-286-NA)

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Pathways for Erythropoietin Antibody (AB-286-NA)

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Blogs on Erythropoietin.

HIF-2 alpha Antibody Throws New Light on EPO Dilemma
The HIF family are heterodimeric, oxygen-sensitive transcription factors comprising an alpha and beta subunit which are normally dissociated in normoxic conditions. Our antibody catalog contains products targeting all the Hypoxia Inducible Factor isof...  Read full blog post.

HIF Antibodies: Beyond HIF-1 alpha
The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the...  Read full blog post.

Hypoxia-Inducible Factors and Homeostasis
Hypoxia-Inducible Factor-1 (HIF-1) is a highly conserved heterodimeric transcription factor. Novus' antibody catalogue contains an extensive range of both HIF-1 alpha and HIF-1 beta, useful for hypoxia, angiogenesis, cancer and many other areas of res...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol EPO