ERManI Antibody

Images

 
Western Blot: ERManI Antibody [NBP1-31448] - Analysis of NBP1-31448 on Hela, with 7.5% SDS PAGE, diluted at 1:1000.
Immunohistochemistry-Paraffin: ERManI Antibody [NBP1-31448] - Sample: Paraffin-embedded mouse kidney. ERManI antibody diluted at 1:500.
Immunohistochemistry-Paraffin: ERManI Antibody [NBP1-31448] - Paraffin-embedded SCC25 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: ERManI Antibody [NBP1-31448] - Sample: Paraffin-embedded mouse liver. ERManI antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.84 mg/ml

Order Details

ERManI Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MAN1B1. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane, Single-pass type II membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAN1B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.84 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERManI Antibody

  • alpha 1,2-mannosidase
  • EC 3.2.1.113
  • endoplasmic reticulum alpha-mannosidase 1
  • endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1
  • endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
  • ER alpha 1,2-mannosidase
  • ER alpha-1,2-mannosidase
  • ER ManI
  • ER mannosidase 1
  • ERMan1
  • Man9GlcNAc2-specific processing alpha-mannosidase
  • Man9GlcNAc2-specific-processing alpha-mannosidase
  • MANA-ER
  • Mannosidase alpha class 1B member 1
  • mannosidase, alpha, class 1B, member 1
  • MRT15
  • RP11-350O14.2

Background

ERManI (Mannosidase, alpha, class 1B, member 1) catalyzes the first mannose-trimming step in maturation of Asn-linked oligosaccharides. It trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2), specifically converting it to Man8GlcNAc isomer B. ERManI may be involved in glycoprotein quality control since it is important to target misfolded glycoproteins for degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAN1B1