ERK5/BMK1 Antibody (3H2)

Images

 
Western Blot: ERK5 Antibody (3H2) [NBP2-03371] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK5 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: ERK5 Antibody (3H2) [NBP2-03371] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERK5.
Flow Cytometry: ERK5 Antibody (3H2) [NBP2-03371] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERK5 antibody, and then analyzed by ...read more
Western Blot: ERK5 Antibody (3H2) [NBP2-03371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
3H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ERK5/BMK1 Antibody (3H2) Summary

Immunogen
Full length human recombinant protein of human MAPK7 (NP_002740) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAPK7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ERK5/BMK1 Antibody (3H2)

  • Big MAP kinase 1
  • BMK1 kinase
  • BMK1
  • BMK-1
  • BMK1EC 2.7.11.24
  • EC 2.7.11
  • ERK5
  • ERK-5
  • ERK5MAPK 7
  • Extracellular signal-regulated kinase 5
  • extracellular-signal-regulated kinase 5
  • MAP kinase 7
  • MAPK7
  • mitogen-activated protein kinase 7
  • PRKM7ERK4

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MAPK7
Entrez