ERK2 Antibody

Images

 
Western Blot: ERK2 Antibody [NB120-14018] - E coli-derived fusion protein as test antigen. Affinity-Purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, EM, ICC/IF
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

ERK2 Antibody Summary

Immunogen
Recombinant fragment, corresponding to amino acids 232-360 of Human ERK2.
Localization
Cytoplasmic
Specificity
ERK2
Clonality
Polyclonal
Host
Chicken
Gene
MAPK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence
Application Notes
ICC: Use at a dilution of 1/200. Western Blot: Use at a dilution of 1/500. Predicted molecular weight: 41 kDa. Optimal dilutions/concentrations should be determined by the end user.
Positive Control
ERK2 Lysate (NBL1-12865)

Reactivity Notes

Cross-reacts with Human, Mouse and Rat.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERK2 Antibody

  • EC 2.7.11
  • EC 2.7.11.24
  • ERK
  • ERK2
  • ERK-2
  • ERK2MAP kinase isoform p42
  • ERT1
  • Extracellular signal-regulated kinase 2
  • MAP kinase 1
  • MAP kinase 2
  • MAPK 1
  • MAPK1
  • MAPK2
  • mitogen-activated protein kinase 1
  • Mitogen-activated protein kinase 2
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • p42-MAPK
  • PRKM1
  • PRKM1MAPK 2
  • PRKM2
  • protein tyrosine kinase ERK2

Background

ERK2 is activated by MEK2, which phosphorylates neighbouring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal componant of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERK2 Antibody (NB120-14018) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ERK2 Antibody (NB120-14018)

Discover related pathways, diseases and genes to ERK2 Antibody (NB120-14018). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for ERK2 Antibody (NB120-14018)

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Blogs on ERK2.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. Enc...  Read full blog post.

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Calculators

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Bioinformatics

Gene Symbol MAPK1
Entrez