eRF1 Antibody (2E3)

Images

 
Western Blot: eRF1 Antibody (2E3) [NBP2-00740] - Analysis of extracts (35ug) from 9 different cell lines by using anti-eRF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549:human; COS7: monkey; Jurkat: ...read more
Flow Cytometry: eRF1 Antibody (2E3) [NBP2-00740] - Analysis of Jurkat cells, using anti-eRF1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: eRF1 Antibody (2E3) [NBP2-00740] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Flow Cytometry: eRF1 Antibody (2E3) [NBP2-00740] - Analysis of Hela cells, using anti-eRF1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, MkSpecies Glossary
Applications WB, Flow
Clone
2E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

eRF1 Antibody (2E3) Summary

Immunogen
Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ETF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for eRF1 Antibody (2E3)

  • D5S1995
  • ERF1eRF1
  • eukaryotic peptide chain release factor subunit 1
  • Eukaryotic release factor 1
  • eukaryotic translation termination factor 1
  • polypeptide chain release factor 1
  • Protein Cl1
  • RF1ERF
  • sup45 (yeast omnipotent suppressor 45) homolog-like 1
  • SUP45L1
  • TB3-1MGC111066

Background

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-14951
Species: Hu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
AF1443
Species: Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP1-89064
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-22169
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-47442
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80306
Species: Hu
Applications: WB, IHC, IHC-P
MAB4614
Species: Hu
Applications: Flow, CyTOF-ready
NBP2-07799
Species: Hu
NBP2-60655
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB120-6125
Species: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
AF5415
Species: Hu
Applications: WB, IHC, ICC
NBP2-00740
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, Flow

Publications for eRF1 Antibody (NBP2-00740) (0)

There are no publications for eRF1 Antibody (NBP2-00740).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for eRF1 Antibody (NBP2-00740) (0)

There are no reviews for eRF1 Antibody (NBP2-00740). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for eRF1 Antibody (NBP2-00740) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional eRF1 Products

Bioinformatics Tool for eRF1 Antibody (NBP2-00740)

Discover related pathways, diseases and genes to eRF1 Antibody (NBP2-00740). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for eRF1 Antibody (NBP2-00740)

Discover more about diseases related to eRF1 Antibody (NBP2-00740).
 

Pathways for eRF1 Antibody (NBP2-00740)

View related products by pathway.

PTMs for eRF1 Antibody (NBP2-00740)

Learn more about PTMs related to eRF1 Antibody (NBP2-00740).

Blogs on eRF1

There are no specific blogs for eRF1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our eRF1 Antibody (2E3) and receive a gift card or discount.

Bioinformatics

Gene Symbol ETF1
Entrez