ERCC1 Antibody (1A3)

Images

 
Western Blot: ERCC1 Antibody (1A3) [NBP1-47733] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (1A3) [NBP1-47733] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded thyroid tissue using anti-ERCC1 mouse monoclonal antibody.
Flow Cytometry: ERCC1 Antibody (1A3) [NBP1-47733] - HEK293T cells transfected with either pCMV6-ENTRY ERCC1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-ERCC1 mouse monoclonal, and then ...read more
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded bladder tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Kidney tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded lung tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded Ovary tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded pancreas tissue using anti-ERCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: ERCC1 Antibody (1A3) [NBP1-47733] - Staining of paraffin-embedded prostate tissue using anti-ERCC1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ERCC1 Antibody (1A3) Summary

Immunogen
Protein expressed in 293T cell transfected with human ERCC1 expression vector
Specificity
This antibody is specific for Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1), transcript variant 2.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ERCC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ERCC1 Antibody (1A3)

  • COFS4
  • DNA excision repair protein ERCC-1
  • ERCC1
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence)
  • RAD10
  • UV20

Background

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ERCC1 Antibody (NBP1-47733)

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53BP1 - DNA damage is no fun
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Using PCNA as an Antibody Marker
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Bioinformatics

Gene Symbol ERCC1
Entrez
Uniprot