Erbin Antibody - BSA Free

Western Blot: Erbin Antibody [NBP2-13968] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ERBIN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: Erbin Antibody [NBP2-13968] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968] - Staining of human breast shows strong membranous positivity in myoepithelial cells.

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968] - Staining of human gastrointestinal shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Erbin Antibody [NBP2-13968] - Staining of human salivary gland shows moderate membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Erbin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Erbin Antibody - BSA Free (NBP2-13968) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Erbin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: WHSKQNPQIDHASFPPQLLPRSESTENQSYAKHSANMNFSNHNNVRANTAYHLHQRLGPARHGEMWAISPNDRLIPAVTRSTIQR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERBIN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  Erbin Protein (NBP2-13968PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Erbin Antibody - BSA Free

• Densin-180-like protein
• erbb2 interacting protein
• Erbb2-interacting proteinErbin
• ERBB2IP
• Erbin
• ERBINLAP2protein LAP2
• KIAA1225

Background

The ERBB2IP gene encodes a LAP2 protein that exists in nine isoforms: Isoform 1: 1,412 amino acids long, 158 kDA; isoform 2: 1,371 amino acids long, 153 kDA; isoform 3: 1,360 amino acids long, 152 kDA; isoform 4: 1,346 amino acids, 151 kDA; isoform 5: 1,356 amino acids long, 152 kDA; isoform 6: 1,340 amino acids long, 150 kDA; isoform 7: 1,302 amino acids long, 146 kDA; isoform 8: 1,419 amino acids long, 159 kDA; isoform 9 is 1,367 amino acids long at 153 kDA. ERBB2IP functions as an adapter for the receptor ERBB2, and through binding of this receptor, it contributes to the stabilization of this unphosphorylated state. ERBB2IP participates in the NOD pathway, TGF-beta receptor signaling pathway, signal transduction, and with NLR proteins. It is known to interact with genes PKP4, SMAD3, ERBB2, ZFYVE9, and CTNNB1. ERBB2IP is associated with breast cancer, carcinoma, cervical cancer, crohn's disease, and epidermolysis bullosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Erbin Antibody (NBP2-13968)(2)

Publications using NBP2-13968

• Yang, T;Sim, KY;Ko, GH;Ahn, JS;Kim, HJ;Park, SG; FAM167A is a key molecule to induce BCR-ABL-independent TKI resistance in CML via noncanonical NF-kappa B signaling activation Journal of experimental & clinical cancer research : CR [PMID: 35241148]
• Li H, Zhou W, Li L et al. Inhibition of Neddylation Modification Sensitizes Pancreatic Cancer Cells to Gemcitabine. Neoplasia 2017-05-20 [PMID: 28535453] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: ERBIN