ErbB2/Her2 [p Tyr1248] Antibody

Images

 
Western Blot: HER2 Phosphospecific Antibody [NB100-92548] - Western blot (WB) analysis of p-HER2 (Y1248) antibody in extracts from SK-OV3 cells treated with EGF.
Immunohistochemistry: HER2 Phosphospecific Antibody [NB100-92548] - Immunohistochemistry (IHC) analyzes of p-HER2 (Y1248) antibody in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related ErbB2/Her2 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92548
    • Availability
      Product Discontinued

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ErbB2/Her2 [p Tyr1248] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human ErbB2/HER2 around the phosphorylation site of Tyrosine 1248.
Modification
p Tyr1248
Specificity
p-HER2 (Y1248) detects endogenous levels of p-HER2 (Y1248) protein.
Clonality
Polyclonal
Host
Rabbit
Gene
ERBB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of p-HER2 (Y1248) pAb in extracts from SK-OV3 cells treated with EGF.
Immunohistochemistry (IHC) analyzes of p-HER2 (Y1248) pAb in paraffin-embedded human breast carcinoma tissue. Testing on frozen sections has been reported negative (customer feeback).
Theoretical MW
138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB100-92548 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ErbB2/Her2 [p Tyr1248] Antibody

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Neu, a 185 kDa glycoprotein, undergoes transactivation upon heterodimerizaion with other EGF receptor family members. Neu heterodimerization with ErbB-3 recruits heregulin, which induces phosphoinositide (PI) 3-kinase activation. Activation of Neu potentiates tumor cell motility and protease secretion and invasion, and also modulates cell cycle checkpoint function, DNA repair and apoptotic responses. Amplification and/or overexpression of Neu occurs in 20-30% of breast carcinomas. Measurement of increased Neu expression can be a predictor of disease prognosis. Neu may also prove to bea promising target for therapeutic agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB2/Her2 Antibody (NB100-92548)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol

FAQs for ErbB2/Her2 Antibody (NB100-92548). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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Bioinformatics

Gene Symbol ERBB2
Entrez