Western blot (WB) analysis of p-HER2 (Y1248) pAb in extracts from SK-OV3 cells treated with EGF. Immunohistochemistry (IHC) analyzes of p-HER2 (Y1248) pAb in paraffin-embedded human breast carcinoma tissue. Testing on frozen sections has been reported negative (customer feeback).
138 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Neu, a 185 kDa glycoprotein, undergoes transactivation upon heterodimerizaion with other EGF receptor family members. Neu heterodimerization with ErbB-3 recruits heregulin, which induces phosphoinositide (PI) 3-kinase activation. Activation of Neu potentiates tumor cell motility and protease secretion and invasion, and also modulates cell cycle checkpoint function, DNA repair and apoptotic responses. Amplification and/or overexpression of Neu occurs in 20-30% of breast carcinomas. Measurement of increased Neu expression can be a predictor of disease prognosis. Neu may also prove to bea promising target for therapeutic agents.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for ErbB2/Her2 Antibody (NB100-92548). (Showing 1 - 1 of 1 FAQ).
I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
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