ErbB2/Her2 Antibody (S-495)

Images

 
Western Blot: ErbB2/Her2 Antibody (S-495) [NBP1-72166] - ErbB2/HER2 Antibody (S-495) The whole cell lysates from SK-BR-3 were resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an Immunoblotting ...read more
Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (S-495) [NBP1-72166] - SK-BR-3 cells were fixed and immunoblotted by Mouse anti Her-2 incubation at a 1:250 dilution.

Product Details

Summary
Product Discontinued
View other related ErbB2/Her2 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-72166
    • Availability
      Product Discontinued

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ErbB2/Her2 Antibody (S-495) Summary

Immunogen
A synthetic peptide corresponding to the C-terminus of ErbB2/HER2 protein.
Specificity
This antibody recognizes ErbB2/HER2 protein. It cross-reacts with human, rat and mouse. The other species was not tested.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ERBB2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Sterile TBS (pH 7.2) with antibody stabilizer
Preservative
No Preservative
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Western Blot 0.1-1 ug/ml
  • ELISA 0.01-0.1 ug/ml
  • Flow Cytometry 5-10 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2-5 ug/ml
  • Immunoprecipitation 2-5 ug/ml
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-72166.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for ErbB2/Her2 Antibody (S-495)

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

Oncoprotein Her-2/Neu/ c-erbB-2 belongs to one of the four members of the ErbB receptor family. They are transmembrane receptor-like tyrosine kinases which can be autophosphorylated without ligand binding process when it is overexpressed in breast cancers. Her2 is an important biomarker in breast cancer diagnosis. The antibodies/ heregulin can suppress phosphorylation of HER2 on tyrosine Y1248. Endogenous anti-HER2 antibodies can effectively suppress HER2 kinase activity and downstream signaling to inhibit the transformed phenotype of HER2-expressing tumor cells. Thus, anti-Her2 antibodies have been used widely in the treatment of the breast cancer and other carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB2/Her2 Antibody (NBP1-72166)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ErbB2/Her2 Antibody (NBP1-72166). (Showing 1 - 2 of 2 FAQ).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22
  2. May we ask if it is possible to confirm if NBP1-72166 contains any other protein besides antibodies?
    • The lab confirmed that no other proteins other than antibodies exists in our ErbB2/HER2 antibody # NB110-61008. NB110-61008 is one of our best sellers.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ERBB2
Entrez
Uniprot