ER alpha/NR3A1 Antibody

Images

 
Western Blot: Estrogen Receptor alpha Antibody [NB300-1065] - analysis of Estrogen Receptor alpha protein in MCF cell lysate with the anti ER-alpha (paired 167) antibody
Western Blot: Estrogen Receptor alpha Antibody [NB300-1065] - Western blot analysis of ER-alpha (Paired 167) in Jurkat whole cell lysate with anti- ER-alpha (Paired 167) at 1 ig/ml

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Gt, ShSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

ER alpha/NR3A1 Antibody Summary

Immunogen
Synthetic peptide: GGRERLASTNDK, corresponding to amino acids 160-171 of Human Estrogen Receptor (alpha).
Localization
Nuclear
Specificity
Estrogen Receptor alpha
Predicted Species
Porcine (91%), Canine (91%), Goat (91%), Sheep (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ESR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Application Notes
This antibody can be used for Western Blot. Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in ELISA and Immunoprecipitation.
Positive Control
MCF-7 Lysate (NB820-59465)

Reactivity Notes

Expected to cross-react with Dog (91% identity with immunogen), Goat (91% identity with immunogen), Pig (91% identity with immunogen), Sheep (91% identity with immunogen) due to sequence identity.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ER alpha/NR3A1 Antibody

  • ER alpha
  • ER
  • Era
  • ER-alpha
  • ESR1
  • ESRESRA
  • Estradiol receptor
  • estrogen receptor 1
  • estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
  • estrogen receptor alpha delta 4 +49 isoform
  • estrogen receptor alpha delta 4*5,6,7*/654 isoform
  • estrogen receptor alpha
  • estrogen receptor
  • NR3A1
  • NR3A1DKFZp686N23123
  • Nuclear receptor subfamily 3 group A member 1

Background

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ESR1
Entrez