Ephrin-A2 Antibody (3E3)

Images

 
Western Blot: Ephrin A2 Antibody (3E3) [NBP2-02810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ephrin A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: Ephrin A2 Antibody (3E3) [NBP2-02810] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2.
Immunohistochemistry-Paraffin: Ephrin A2 Antibody (3E3) [NBP2-02810] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ephrin A2 mouse monoclonal antibody.
Flow Cytometry: Ephrin A2 Antibody (3E3) [NBP2-02810] - Analysis of Jurkat cells, using anti-Ephrin A2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Ephrin-A2 Antibody (3E3) Summary

Immunogen
Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EFNA2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ephrin-A2 Antibody (3E3)

  • Cek7-L
  • EFNA2
  • ELF-1
  • EPH-related receptor tyrosine kinase ligand 6
  • EphrinA2
  • Ephrin-A2
  • EPLG6HEK7 ligand
  • HEK7-L
  • HEK7-ligand
  • LERK6
  • LERK6LERK-6
  • ligand of eph-related kinase 6

Background

This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EFNA2
Entrez