EpCAM/TROP1 Antibody

Images

 
Western Blot: EpCAM/TROP1 Antibody [NBP1-33439] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EpCAM antibody [N3C3] ) diluted at 1:5000.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody [NBP1-33439] - NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: EpCAM/TROP1 Antibody [NBP1-33439] - 293T lysates (Lane A), A431 lysates (Lane B), H1299 lysates (Lane C) and Hela cells (Lane D) using NBP1-33439 at a dilution of 1:1000.
Western Blot: EpCAM/TROP1 Antibody [NBP1-33439] - (30 ug of whole cell lysate) A: THP-1 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: EpCAM/TROP1 Antibody [NBP1-33439] - A. 30 ug Molt-4 whole cell extract 12 % SDS-PAGEEpCAM antibody [N3C3] ) dilution: 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.81 mg/ml

Order Details

EpCAM/TROP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EpCAM. The exact sequence is proprietary.
Localization
Membrane, Single-pass type I membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPCAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (81%), Rat (83%), Sheep (82%),Rhesus Monkey, Bovine (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.81 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EpCAM/TROP1 Antibody

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Background

CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for EpCAM/TROP1 Antibody (NBP1-33439). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

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Bioinformatics

Gene Symbol EPCAM
Entrez
Uniprot