EpCAM/TROP1 Antibody (4G10)

Images

 
Western Blot: EpCAM/TROP1 Antibody (4G10) [NBP1-47729] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EpCAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (4G10) [NBP1-47729] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY EpCAM.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (4G10) [NBP1-47729] - Staining of paraffin-embedded pancreas using anti-EpCAM mouse monoclonal antibody.
Western Blot: EpCAM/TROP1 Antibody (4G10) [NBP1-47729] - EpCAM antibody(4G10) at 1:500 dilution + MCF7 cell lysate
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (4G10) [NBP1-47729] - Staining of paraffin-embedded breast using anti-EpCAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (4G10) [NBP1-47729] - Staining of paraffin-embedded Carcinoma of lung using anti-EpCAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (4G10) [NBP1-47729] - Staining of paraffin-embedded kidney using anti-EpCAM mouse monoclonal antibody.
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (4G10) [NBP1-47729] - Staining of paraffin-embedded lung using anti-EpCAM mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
4G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

EpCAM/TROP1 Antibody (4G10) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 24-265 of human EpCAM
Specificity
This antibody is specific for Homo sapiens epithelial cell adhesion molecule (EPCAM).
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
EPCAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EpCAM/TROP1 Antibody (4G10)

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Background

EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EpCAM/TROP1 Antibody (NBP1-47729). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol EPCAM
Entrez